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2030-12-31
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HUMAN PEGIVIRUS-1 DETECTION AND GENOTYPING IN BRAZILIAN PATIENTS WITH FULMINANT HEPATITIS
Author
Silva, Anielly Sarana da
Campos, Gabriel Montenegro de
Villanova, Marcia Guimarães
Bezerra, Rafael Dos Santos
Santiago, Luciana Maria Mendes
Haddad, Rodrigo
Covas, Dimas Tadeu
Giovanetti, Marta
Alcantara, Luiz Carlos Junior
Elias, Maria Carolina
Sampaio, Sandra Coccuzzo
Kashima, Simone
Slavov, Svetoslav Nanev
Campos, Gabriel Montenegro de
Villanova, Marcia Guimarães
Bezerra, Rafael Dos Santos
Santiago, Luciana Maria Mendes
Haddad, Rodrigo
Covas, Dimas Tadeu
Giovanetti, Marta
Alcantara, Luiz Carlos Junior
Elias, Maria Carolina
Sampaio, Sandra Coccuzzo
Kashima, Simone
Slavov, Svetoslav Nanev
Affilliation
Blood Center of Ribeirão Preto. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil.
Blood Center of Ribeirão Preto. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil.
Department of Gastroenterology. University Hospital. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil.
Blood Center of Ribeirão Preto. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil.
Blood Center of Ribeirão Preto. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil.
Faculty of Ceilândia. University of Brasília. Brasília, DF, Brazil.
Blood Center of Ribeirão Preto. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil.
Instituto Rene Rachou. Fundação Oswaldo Cruz. Belo Horizonte, MG, Brazil / Sciences and Technologies for Sustainable Development and One Health. Università Campus Bio-Medico di Roma. Rome, Italy.
Sciences and Technologies for Sustainable Development and One Health. Università Campus Bio-Medico di Roma. Rome, Italy.
Center for Scientific Development. Butantan Institute. São Paulo, SP, Brazil.
Center for Scientific Development. Butantan Institute. São Paulo, SP, Brazil.
Blood Center of Ribeirão Preto. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil.
Blood Center of Ribeirão Preto. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil / Center for Scientific Development. Butantan Institute. São Paulo, SP, Brazil.
Blood Center of Ribeirão Preto. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil.
Department of Gastroenterology. University Hospital. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil.
Blood Center of Ribeirão Preto. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil.
Blood Center of Ribeirão Preto. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil.
Faculty of Ceilândia. University of Brasília. Brasília, DF, Brazil.
Blood Center of Ribeirão Preto. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil.
Instituto Rene Rachou. Fundação Oswaldo Cruz. Belo Horizonte, MG, Brazil / Sciences and Technologies for Sustainable Development and One Health. Università Campus Bio-Medico di Roma. Rome, Italy.
Sciences and Technologies for Sustainable Development and One Health. Università Campus Bio-Medico di Roma. Rome, Italy.
Center for Scientific Development. Butantan Institute. São Paulo, SP, Brazil.
Center for Scientific Development. Butantan Institute. São Paulo, SP, Brazil.
Blood Center of Ribeirão Preto. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil.
Blood Center of Ribeirão Preto. Faculty of Medicine of Ribeirão Preto. University of São Paulo. Ribeirão Preto, SP, Brazil / Center for Scientific Development. Butantan Institute. São Paulo, SP, Brazil.
Abstract
Fulminant hepatitis is a severe clinical disease characterized by a marked decline in liver function and encephalopathy. In a previous survey, using metagenomics in a group of 27 patients with this clinical condition, we observed an expressive quantity of reads of the Human pegivirus-1 (HPgV-1). Therefore, the objective of this study was to evaluate the frequency, molecular features, and HPgV-1 circulating genotypes in patients with fulminant hepatitis. After testing the collected plasma samples, we discovered twelve samples (44.4%) that were positive for HPgV-1 RNA (using both real-time and nested PCR). The positive samples presented a mean cycle threshold (Ct) of 28.5 (±7.3). Genotyping assignments revealed that all HPgV-1 positive samples belonged to the HPgV-1 genotype 2 (both subgenotypes 2A and 2B were identified). Although HPgV-1 is considered a commensal virus, little is known regarding its prevalence and genotypes in cases of fulminant hepatitis. More research is needed to understand whether HPgV-1 can be implicated in clinical disorders and infectious diseases.
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