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https://www.arca.fiocruz.br/handle/icict/60983
POTENT ACTIVITY OF A HIGH CONCENTRATION OF CHEMICAL OZONE AGAINST ANTIBIOTIC-RESISTANT BACTERIA
Bactéria patogênica
Resistência antimicrobiana
SEM
Patógenos ESKAPE
Actividade antimicrobiana
Pathogenic bacteria
Antimicrobial resistance
SEM
ESKAPE pathogens
Antimicrobial activity
Author
Affilliation
Oswaldo Cruz Foundation. Center for Technological Development in Health. Rio de Janeiro, RJ, Brasil / Oswaldo Cruz Foundation. Oswaldo Cruz Institute. National Institute of Science and Technology for Innovation in Neglected Population Diseases. Rio de Janeiro, RJ, Brasil.
Federal Fluminense University. Biology Institute. Department of Molecular and Cellular Biology. Post-Graduation Program in Science and Biotechnology. Niterói, RJ, Brazil.
Oswaldo Cruz Foundation. Center for Technological Development in Health. Rio de Janeiro, RJ, Brasil / Oswaldo Cruz Foundation. Oswaldo Cruz Institute. National Institute of Science and Technology for Innovation in Neglected Population Diseases. Rio de Janeiro, RJ, Brasil.
Oswaldo Cruz Foundation. Center for Technological Development in Health. Rio de Janeiro, RJ, Brasil / Oswaldo Cruz Foundation. Oswaldo Cruz Institute. National Institute of Science and Technology for Innovation in Neglected Population Diseases. Rio de Janeiro, RJ, Brasil.
Oswaldo Cruz Foundation. National Institute for Quality Control in Health. Microbiology Department. Rio de Janeiro, RJ, Brasil.
Oswaldo Cruz Foundation. Oswaldo Cruz Institute. Laboratory of Cellular and Ultrastructure. Rio de Janeiro, RJ, Brasil.
Oswaldo Cruz Foundation. Center for Technological Development in Health. Rio de Janeiro, RJ, Brasil / Oswaldo Cruz Foundation. Oswaldo Cruz Institute. National Institute of Science and Technology for Innovation in Neglected Population Diseases. Rio de Janeiro, RJ, Brasil.
Federal Fluminense University. Biology Institute. Department of Molecular and Cellular Biology. Post-Graduation Program in Science and Biotechnology. Niterói, RJ, Brazil.
Oswaldo Cruz Foundation. Center for Technological Development in Health. Rio de Janeiro, RJ, Brasil / Oswaldo Cruz Foundation. Oswaldo Cruz Institute. National Institute of Science and Technology for Innovation in Neglected Population Diseases. Rio de Janeiro, RJ, Brasil.
Oswaldo Cruz Foundation. Center for Technological Development in Health. Rio de Janeiro, RJ, Brasil / Oswaldo Cruz Foundation. Oswaldo Cruz Institute. National Institute of Science and Technology for Innovation in Neglected Population Diseases. Rio de Janeiro, RJ, Brasil.
Oswaldo Cruz Foundation. National Institute for Quality Control in Health. Microbiology Department. Rio de Janeiro, RJ, Brasil.
Oswaldo Cruz Foundation. Oswaldo Cruz Institute. Laboratory of Cellular and Ultrastructure. Rio de Janeiro, RJ, Brasil.
Oswaldo Cruz Foundation. Center for Technological Development in Health. Rio de Janeiro, RJ, Brasil / Oswaldo Cruz Foundation. Oswaldo Cruz Institute. National Institute of Science and Technology for Innovation in Neglected Population Diseases. Rio de Janeiro, RJ, Brasil.
Abstract
Background: Health care-associated infections (HAIs) are a significant public health problem worldwide, favoring multidrug-resistant (MDR) microorganisms. The SARS-CoV-2 infection was negatively associated with the increase in antimicrobial resistance, and the ESKAPE group had the most significant impact on HAIs. The study evaluated the bactericidal effect of a high concentration of O3 gas on some reference and ESKAPE bacteria. Material and Methods: Four standard strains and four clinical or environmental MDR strains were exposed to elevated ozone doses at different concentrations and times. Bacterial inactivation (growth and cultivability) was investigated using colony counts and resazurin as metabolic indicators. Scanning electron microscopy (SEM) was performed. Results: The culture exposure to a high level of O3 inhibited the growth of all bacterial strains tested with a statistically significant reduction in colony count compared to the control group. The cell viability of S. aureus (MRSA) (99.6%) and P. aeruginosa (XDR) (29.2%) was reduced considerably, and SEM showed damage to bacteria after O3 treatment Conclusion: The impact of HAIs can be easily dampened by the widespread use of ozone in ICUs. This product usually degrades into molecular oxygen and has a low toxicity compared to other sanitization products. However, high doses of ozone were able to interfere with the growth of all strains studied, evidencing that ozone-based decontamination approaches may represent the future of hospital cleaning methods.
Keywords in Portuguese
OzônioBactéria patogênica
Resistência antimicrobiana
SEM
Patógenos ESKAPE
Actividade antimicrobiana
Keywords
OzonePathogenic bacteria
Antimicrobial resistance
SEM
ESKAPE pathogens
Antimicrobial activity
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