Author | Calvet, Guilherme | |
Author | Ogrzewalska, Maria | |
Author | Tassinari, Wagner | |
Author | Guaraldo, Lusiele | |
Author | Resende, Paola | |
Author | Fuller, Trevon | |
Author | Penetra, Stephanie | |
Author | Borges, Michele | |
Author | Pina-Costa, Anielle | |
Author | Martins, Ezequias | |
Author | Moraes, Isabella | |
Author | Santos, Heloisa | |
Author | Damasceno, Luana | |
Author | Medeiros-Filho, Fernando | |
Author | Espindola, Otavio | |
Author | Mota, Fernando | |
Author | Nacife, Valéria | |
Author | Pauvolid-Corrêa, Alex | |
Author | Whitworth, Jimmy | |
Author | Smith, Chris | |
Author | Siqueira, Marilda | |
Author | Brasil, Patrícia | |
Access date | 2023-08-25T23:23:40Z | |
Available date | 2023-08-25T23:23:40Z | |
Document date | 2023 | |
Citation | CALVET, Guilherme et al. Accuracy of saliva for SARS-CoV-2 detection in outpatients and their household contacts during the circulation of the Omicron variant of concern. BMC Infectious Diseases, v. 13, p. 1-9, May 2023. | en_US |
ISSN | 1471-2334 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/60234 | |
Description | A Rede Genômica Fiocruz é formada por especialistas de todas as unidades da Fundação no país e de institutos parceiros que se empenham diariamente em gerar dados mais robustos sobre o comportamento do SARS-Cov-2 e contribuir para um melhor preparo do país no enfrentamento da pandemia em termos de diagnóstico mais precisos e vacinas eficazes. Saiba mais sobre a Rede Genômica Fiocruz em: http://www.genomahcov.fiocruz.br/ | |
Sponsorship | This work was funded by the Carlos Chagas Filho Foundation for Research Support of the State of Rio de Janeiro (FAPERJ) [FAPERJ/E26/201.379/2021 JCNE (G.C), [FAPERJ/E‑26/210.146/2020 COVID (P.B); FAPERJ/E‑26/200.935/2022 CNE (P.B), FAPERJ/E26/210.196/2020 (M.S)]; Department of Science and Technology (DECIT) of the Brazilian Ministry of Health (25000.072811/2016‑19) (P.B), The National Council for Scientific and Technological Development (CNPq), Ministry of Science, Technology and Innovation (MCT) [402457/2020‑0] (M.S); INOVA Oswaldo Cruz Foundation (Fiocruz) [VPPCB‑005‑FIO‑20‑2] (M.S); UK Medical Research Council [MR/V033530/1] (J.W and C.S). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. | en_US |
Language | eng | en_US |
Publisher | Springer Nature | en_US |
Rights | open access | en_US |
Title | Accuracy of saliva for SARS-CoV-2 detection in outpatients and their household contacts during the circulation of the Omicron variant of concern | en_US |
Type | Article | |
DOI | 10.1186/s12879-023-08271-3 | |
Abstract | Background While nasopharyngeal (NP) swabs are considered the gold standard for severe acute respiratory coronavirus 2 (SARS‑CoV‑2) real‑time reverse transcriptase‑polymerase chain reaction (RT‑PCR) detection, several studies have shown that saliva is an alternative specimen for COVID‑19 diagnosis and screening. Methods To analyze the utility of saliva for the diagnosis of COVID‑19 during the circulation of the Omicron variant, participants were enrolled in an ongoing cohort designed to assess the natural history of SARS‑CoV‑2 infection in adults and children. Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and Cohen’s kappa coefficient were calculated to assess diagnostic performance. Results Overall, 818 samples were collected from 365 outpatients from January 3 to February 2, 2022. The median age was 32.8 years (range: 3–94 years). RT‑PCR for SARS‑CoV‑2 was confirmed in 97/121 symptomatic patients (80.2%) and 62/244 (25.4%) asymptomatic patients. Substantial agreement between saliva and combined nasopharyngeal/ oropharyngeal samples was observed with a Cohen’s kappa value of 0.74 [95% confidence interval (CI): 0.67–0.81]. Sensitivity was 77% (95% CI: 70.9–82.2), specificity 95% (95% CI: 91.9–97), PPV 89.8% (95% CI: 83.1–94.4), NPV 87.9% (95% CI: 83.6–91.5), and accuracy 88.5% (95% CI: 85.0‑91.4). Sensitivity was higher among samples collected from symptomatic children aged three years and older and adolescents [84% (95% CI: 70.5–92)] with a Cohen’s kappa value of 0.63 (95% CI: 0.35–0.91). Conclusions Saliva is a reliable fluid for detecting SARS‑CoV‑2, especially in symptomatic children and adolescents during the circulation of the Omicron variant. | en_US |
Affilliation | Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Diseases. Acute Febrile Illnesses Laboratory. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Oswaldo Cruz Foundation. Oswaldo Cruz Institute. Rio de Janeiro, RJ, Brazil / SARS-CoV-2 National Reference Laboratory. Brazilian Ministry of Health (MoH) and Regional Reference Laboratory in Americas. Pan-American Health Organization. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Federal Rural University of Rio de Janeiro. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Diseases. Acute Febrile Illnesses Laboratory. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Oswaldo Cruz Foundation. Oswaldo Cruz Institute. Rio de Janeiro, RJ, Brazil / SARS-CoV-2 National Reference Laboratory. Brazilian Ministry of Health (MoH) and Regional Reference Laboratory in Americas. Pan-American Health Organization. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Diseases. Acute Febrile Illnesses Laboratory. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Diseases. Acute Febrile Illnesses Laboratory. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Diseases. Acute Febrile Illnesses Laboratory. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Diseases. Acute Febrile Illnesses Laboratory. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Diseases. Acute Febrile Illnesses Laboratory. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Diseases. Acute Febrile Illnesses Laboratory. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Diseases. Acute Febrile Illnesses Laboratory. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Diseases. Acute Febrile Illnesses Laboratory. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Diseases. Acute Febrile Illnesses Laboratory. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Diseases. Acute Febrile Illnesses Laboratory. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Oswaldo Cruz Foundation. Oswaldo Cruz Institute. Rio de Janeiro, RJ, Brazil / SARS-CoV-2 National Reference Laboratory. Brazilian Ministry of Health (MoH) and Regional Reference Laboratory in Americas. Pan-American Health Organization. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Oswaldo Cruz Foundation. Oswaldo Cruz Institute. Rio de Janeiro, RJ, Brazil / SARS-CoV-2 National Reference Laboratory. Brazilian Ministry of Health (MoH) and Regional Reference Laboratory in Americas. Pan-American Health Organization. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Oswaldo Cruz Foundation. Oswaldo Cruz Institute. Rio de Janeiro, RJ, Brazil / SARS-CoV-2 National Reference Laboratory. Brazilian Ministry of Health (MoH) and Regional Reference Laboratory in Americas. Pan-American Health Organization. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Departments of Clinical Research and Epidemiology and Public Health. London School of Hygiene and Tropical Medicine. London, UK. | en_US |
Affilliation | Departments of Clinical Research and Epidemiology and Public Health. London School of Hygiene and Tropical Medicine. London, UK. | en_US |
Affilliation | Oswaldo Cruz Foundation. Oswaldo Cruz Institute. Rio de Janeiro, RJ, Brazil / SARS-CoV-2 National Reference Laboratory. Brazilian Ministry of Health (MoH) and Regional Reference Laboratory in Americas. Pan-American Health Organization. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Diseases. Acute Febrile Illnesses Laboratory. Rio de Janeiro, RJ, Brazil. | en_US |
Subject | COVID‑19 | en_US |
Subject | SARS‑CoV‑2 | en_US |
Subject | Omicron variant | en_US |
Subject | Saliva | en_US |
Subject | Accuracy | en_US |