Author | Bernardes, Wilma Patrícia de Oliveira Santos | |
Author | Santos, Thais Garcia | |
Author | Fernandes, Nubia Monteiro Gonçalves Soares | |
Author | Silva, Thais Bárbara de Souza | |
Author | Westin, Mateus | |
Author | Simões, Taynãna César | |
Author | Silva, Eduardo Fernandes e | |
Author | Alves, Breno Magalhães | |
Author | Molina, Israel | |
Author | Melo, Mariana de Carvalho | |
Author | Monte Neto, Rubens Lima do | |
Author | Pereira, Rosiane Aparecida da Silva | |
Author | Alves, Pedro Augusto | |
Author | Fonseca, Cristina Toscano | |
Access date | 2023-08-10T18:17:37Z | |
Available date | 2023-08-10T18:17:37Z | |
Document date | 2023 | |
Citation | BERNARDES, Wilma Patrícia de Oliveira Santos et al. Comparison of diagnostic performance of RT-qPCR, RT-LAMP and IgM/IgG rapid tests for detection of SARS-CoV-2 among healthcare workers in Brazil. J Infect Public Health, v. 16, n. 7, p. 1081-1088, 2023. doi.org/10.1016/j.jiph.2023.05.009. | en_US |
ISSN | 1876-035X | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/60039 | |
Language | eng | en_US |
Publisher | Elsevier. | en_US |
Rights | restricted access | en_US |
Title | Comparison of diagnostic performance of RT-qPCR, RT-LAMP and IgM/IgG rapid tests for detection of SARS-CoV-2 among healthcare workers in Brazil | en_US |
Type | Article | en_US |
Abstract | Background: COVID-19 has become a major public health problem after the outbreak caused by SARS-CoV-2
virus. Great efforts to contain COVID-19 transmission have been applied worldwide. In this context, accurate
and fast diagnosis is essential.
Methods: In this prospective study, we evaluated the clinical performance of three different RNA-basedmolecular tests – RT-qPCR (Charité protocol), RT-qPCR (CDC (USA) protocol) and RT-LAMP – and one rapid test for detecting anti-SARS-CoV-2 IgM and IgG antibodies.
Results: Our results demonstrate that RT-qPCR using the CDC (USA) protocol is the most accurate diagnostic test among those evaluated, while oro-nasopharyngeal swabs are the most appropriate biological sample.RT-LAMP was the RNA-based molecular test with lowest sensitivity while the serological test presented the lowest sensitivity among all evaluated tests, indicating that the latter test is not a good predictor of disease in the first days after symptoms onset. Additionally, we observed higher viral load in individuals who reported more than 3 symptoms at the baseline. Nevertheless, viral load had not impacted the probability of testing positive for SARS-CoV-2.
Conclusion: Our data indicates that RT-qPCR using the CDC (USA) protocol in oro-nasopharyngeal swabs samples should be the method of choice to diagnosis COVID-19 | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Imunologia de Doenças Virais. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Universidade Federal de Minas Gerais Faculdade de Medicina. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Núcleo de Estudos em Saúde Pública e Envelhecimento. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Núcleo de Estudos em Saúde Pública e Envelhecimento. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Hospital Metropolitano Doutor Célio de Castro. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Triatomíneos e Epidemiologia da Doença de Chagas. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Hospital Metropolitano Doutor Célio de Castro. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biotecnologia Aplicada ao Estudo de Patógenos. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Imunologia de Doenças Virais. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Pesquisa em Biologia e Imunologia de Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brazil | en_US |
Subject | SARS-CoV-2 | en_US |
Subject | RT-LAMP | en_US |
Subject | RT-qPCR | en_US |
Subject | TR DPP® COVID-19 IgM/IgG Bio-Manguinhos | en_US |
Subject | Accuracy | en_US |
Embargo date | 3100-12-31 | |