Author | Almeida-Silva, Fernando | |
Author | Almeida, Marcos de Abreu | |
Author | Rabello, Vanessa Brito de Souza | |
Author | Zancopé-Oliveira, Rosely Maria | |
Author | Baeza, Lilian Cristiane | |
Author | Lamas, Cristiane da Cruz | |
Author | Lima, Marco Antonio | |
Author | Macedo, Priscila Marques de | |
Author | Gutierrez-Galhardo, Maria Clara | |
Author | Almeida-Paes, Rodrigo | |
Author | Freitas, Dayvison Francis Saraiva | |
Access date | 2023-07-31T01:15:00Z | |
Available date | 2023-07-31T01:15:00Z | |
Document date | 2023 | |
Citation | ALMEIDA-SILVA, Fernando et al. Evaluation of Five Non-Culture-Based Methods for the Diagnosis of Meningeal Sporotrichosis. Journal of Fungi, v. 9, n. 5, p. 1-14, Apr. 2023. | en_US |
ISSN | 2309-608X | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/59845 | |
Sponsorship | This study was supported in part by the Programa Jovens Pesquisadores—INI/FIOCRUZ (grant INI-003-FIO-19-2-13) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (405653/2021-2 and 403296/2021-8). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. R.M.Z-O is supported in part by CNPq (308315/2021-9) and Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ) (E-26/200.381/2023). | en_US |
Language | eng | en_US |
Publisher | MDPI | en_US |
Rights | open access | en_US |
Title | Evaluation of Five Non-Culture-Based Methods for the Diagnosis of Meningeal Sporotrichosis | en_US |
Type | Article | en_US |
DOI | 10.3390/jof9050535 | |
Abstract | Sporotrichosis is the main subcutaneous mycosis worldwide. Several complications, including meningeal forms, can be observed in immunocompromised individuals. The sporotrichosis diagnosis is time-consuming due to the culture's limitations. The low fungal burden in cerebrospinal fluid (CSF) samples is another important drawback in the diagnosis of meningeal sporotrichosis. Molecular and immunological tests can improve the detection of Sporothrix spp. in clinical specimens. Therefore, the following five non-culture-based methods were evaluated for the detection of Sporothrix spp. in 30 CSF samples: (i) species-specific polymerase chain reaction (PCR); (ii) nested PCR; (iii) quantitative PCR; (iv) enzyme-linked immunosorbent assay (ELISA) for IgG detection; and (v) ELISA for IgM detection. The species-specific PCR was unsuccessful in the diagnosis of the meningeal sporotrichosis. The other four methods presented substantial levels of sensitivity (78.6% to 92.9%) and specificity (75% to 100%) for the indirect detection of Sporothrix spp. Both DNA-based methods presented similar accuracy (84.6%). Both ELISA methods were concomitantly positive only for patients with sporotrichosis and clinical signs of meningitis. We suggest that these methods should be implemented in clinical practice to detect Sporothrix spp. in CSF early, which may optimize treatment, augment the chances of a cure, and improve the prognosis of affected individuals. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Universidade Estadual do Oeste do Paraná. Centro de Ciências Médicas e Farmacêuticas. Cascavel, PR, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Serviço Médico. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Neuroinfecções. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatologia Infecciosa. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatologia Infecciosa. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatologia Infecciosa. Rio de Janeiro, RJ, Brasil. | en_US |
Subject | ELISA | en_US |
Subject | Sporothrix | en_US |
Subject | Cerebrospinal fluid | en_US |
Subject | Immunological diagnosis | en_US |
Subject | Molecular diagnosis | en_US |
Subject | qPCR | en_US |
e-ISSN | 2309-608X | |