Author | Lucena, Aline Castro Rodrigues | |
Author | Ferrarini, Mariana Galvão | |
Author | Oliveira, Willian Klassen de | |
Author | Marcon, Bruna Hilzendeger | |
Author | Morello, Luis Gustavo | |
Author | Alves, Lysangela Ronalte | |
Author | Faoro, Helisson | |
Access date | 2023-07-24T17:19:21Z | |
Available date | 2023-07-24T17:19:21Z | |
Document date | 2023 | |
Citation | LUCENA, Aline Castro Rodrigues et al. Modulation of Klebsiella pneumoniae outer membrane vesicle protein cargo under antibiotic treatment. Biomedicines, v. 11, n. 1515, p. 1-18, 2023. | en_US |
ISSN | 2227-9059 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/59727 | |
Language | por | en_US |
Publisher | MDPI | en_US |
Rights | open access | en_US |
Title | Modulation of Klebsiella pneumoniae outer membrane vesicle protein cargo under antibiotic treatment | en_US |
Type | Article | en_US |
DOI | https://doi.org/10.3390/ biomedicines11061515 | |
Abstract | Klebsiella pneumoniae is a nosocomial pathogen and an important propagator of multidrugresistant (MDR) and extensively drug-resistant (XDR) strains. Like other Gram-negative bacteria, they secrete outer membrane vesicles (OMVs) that distribute virulence and resistance factors. Here, we subjected a K. pneumoniae-XDR to subinhibitory concentrations of meropenem, amikacin, polymyxin B, and a combination of these agents to evaluate changes in the protein cargo of OMVs through liquid chromatography–tandem mass spectrometry (LC-MS/MS). Genome sequencing of the clinical isolate K. pneumoniae strain HCD1 (KpHCD1) revealed the presence of 41 resistance genes and 159 virulence factors. We identified 64 proteins in KpHCD1-OMVs modulated with different antibiotic treatments involved in processing genetic information, environmental information, cell envelope formation, energy metabolism, and drug resistance. The OMV proteome expression profile suggests that OMVs may be associated with pathogenicity, survival, stress response, and resistance dissemination. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. | en_US |
Affilliation | Université de Lyon. Laboratoire de Biométrie et Biologie Évolutive. Villeurbanne, France. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil. / CHU de Quebec Research Center. Department of Microbiology, Infectious Disease and Immunology. Laval University. Quebec, Canada. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Ciências e Tecnologias Aplicadas em Saúde. Curitiba, PR, Brasil. / CHU de Quebec Research Center. Department of Microbiology, Infectious Disease and Immunology. Laval University. Quebec, Canada. | en_US |
Subject | Antibiotic Resistance, Bacterial | en_US |
Subject | Gram-Negative Bacteria | en_US |
Subject | Extracellular Vesicles | en_US |
Subject | Proteomics | en_US |
Subject in Spanish | Farmacorresistencia Bacteriana | en_US |
Subject in Spanish | Bacterias Gramnegativas | en_US |
Subject in Spanish | Vesículas Extracelulares | en_US |
Subject in Spanish | Proteómica | en_US |
Subject in French | Résistance bactérienne aux médicaments | en_US |
Subject in French | Bactéries à Gram négatif | en_US |
Subject in French | Vésicules extracellulaires | en_US |
Subject in French | Protéomique | en_US |
DeCS | Klebsiella pneumoniae | en_US |
DeCS | Farmacorresistência Bacteriana | en_US |
DeCS | Bactérias Gram-Negativas | en_US |
DeCS | Vesículas Extracelulares | en_US |
DeCS | Proteômica | en_US |