Author | Guerra, Mateus Tavares | |
Author | Fonseca, Emerson A. | |
Author | Melo, Flavia M. | |
Author | Andrade, V. A | |
Author | Aguiar, Carla J. | |
Author | Andrade, Lídia Maria | |
Author | Pinheiro, Ana Cristina N. | |
Author | Casteluber, Marisa F. | |
Author | Resende, Rodrigo R. | |
Author | Pinto, Mauro C. X. | |
Author | Fernandes, Simone O. A. | |
Author | Cardoso, Valbert N. | |
Author | Fagundes, Elaine Maria Souza | |
Author | Menezes, Gustavo B. | |
Author | Paula, Ana M. de | |
Author | Nathanson, Michael H. | |
Author | Leite, Maria de Fatima | |
Access date | 2023-07-19T19:38:33Z | |
Available date | 2023-07-19T19:38:33Z | |
Document date | 2011 | |
Citation | GUERRA, Mateus Tavares et al. Mitochondrial calcium regulates rat liver regeneration through the modulation of apoptosis. Hepatology, v. 54, n. 1, p. 296-306. doi: 10.1002/hep.24367. | en_US |
ISSN | 0270-9139 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/59684 | |
Language | eng | en_US |
Publisher | Wolters Kluwer Health | en_US |
Rights | restricted access | en_US |
Title | Mitochondrial Calcium Regulates Rat Liver Regeneration Through the Modulation of Apoptosis | en_US |
Type | Article | en_US |
DOI | 10.1002/hep.24367 | |
Abstract | Subcellular Ca(2+) signals control a variety of responses in the liver. For example, mitochondrial Ca(2+) (Ca(mit)(2+)) regulates apoptosis, whereas Ca(2+) in the nucleus regulates cell proliferation. Because apoptosis and cell growth can be related, we investigated whether Ca(mit)(2+) also affects liver regeneration. The Ca(2+)-buffering protein parvalbumin, which was targeted to the mitochondrial matrix and fused to green fluorescent protein, was expressed in the SKHep1 liver cell line; the vector was called parvalbumin mitochondrial targeting sequence green fluorescent protein (PV-MITO-GFP). This construct properly localized to and effectively buffered Ca(2+) signals in the mitochondrial matrix. Additionally, the expression of PV-MITO-GFP reduced apoptosis induced by both intrinsic and extrinsic pathways. The reduction in cell death correlated with the increased expression of antiapoptotic genes [B cell lymphoma 2 (bcl-2), myeloid cell leukemia 1, and B cell lymphoma extra large] and with the decreased expression of proapoptotic genes [p53, B cell lymphoma 2 associated X protein (bax), apoptotic peptidase activating factor 1, and caspase-6]. PV-MITO-GFP was also expressed in hepatocytes in vivo with an adenoviral delivery system. Ca(mit)(2+) buffering in hepatocytes accelerated liver regeneration after partial hepatectomy, and this effect was associated with the increased expression of bcl-2 and the decreased expression of bax. Conclusion: Together, these results reveal an essential role for Ca(mit)(2+) in hepatocyte proliferation and liver regeneration, which may be mediated by the regulation of apoptosis. (HEPATOLOGY 2011;54:296-306 | en_US |
Affilliation | Department of Physiology and Biophysics. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil/Section of Digestive Diseases. Department of Internal Medicine. Yale University School of Medicine. New Haven, CT, USA | en_US |
Affilliation | Department of Physiology and Biophysics. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Department of Physiology and Biophysics. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Department of Physiology and Biophysics. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Department of Physiology and Biophysics. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil/Izabela Hendrix Metodist Institute. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Department of Physiology and Biophysics. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil/René Rachou Research Center. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Department of Physiology and Biophysics. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Department of Physiology and Biophysics. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Nanobiotechnology Laboratory. Federal University of São João del Rei. São João del Rei, MG, Brazil | en_US |
Affilliation | Department of Physiology and Biophysics. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Radioisotope Laboratory. Department of Clinical and Toxicological Analysis. Faculty of Pharmacy. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Radioisotope Laboratory. Department of Clinical and Toxicological Analysis. Faculty of Pharmacy. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Department of Physiology and Biophysics. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Department of Morphology. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Department of Physics. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Section of Digestive Diseases. Department of Internal Medicine. Yale University School of Medicine. New Haven, CT, USA | en_US |
Affilliation | Department of Physiology and Biophysics. Federal University of Minas Gerais. Belo Horizonte, MG, Brazil/Howard Hughes Medical Institute. | en_US |
Embargo date | 2099-12-31 | |