Author | León, Ileana R. | |
Author | Ferreira, Ana G. C. Neves | |
Author | Valente, Richard H. | |
Author | Mota, Ester Maria | |
Author | Lenzi, Henrique Leonel | |
Author | Perales, Jonas | |
Access date | 2012-12-12T15:21:02Z | |
Available date | 2012-12-12T15:21:02Z | |
Document date | 2007 | |
Citation | LEON, I. R. et al. Improved protein identification efficiency by mass spectrometry using N-terminal chemical derivatization of peptides from Angiostrongylus costaricensis, a nematode with unknown genome. Journal of Mass Spectrometry, v. 42, p. 781-792, 2007. | pt_BR |
ISSN | 1076-5174 | |
URI | https://www.arca.fiocruz.br/handle/icict/5964 | |
Description | Acknowledgements
This study was supported by grants from Conselho Nacional de Desenvolvimento Científico and Tecnológico (CNPq), Fundação
de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ)
and Fundação Oswaldo Cruz-PDTIS, Brazil. IRL thanks Instituto Oswaldo Cruz for her Master's scholarship. | pt_BR |
Language | eng | pt_BR |
Publisher | Wiley | en |
Rights | open access | pt_BR |
Title | Improved protein identification efficiency by mass spectrometry using N-terminal chemical derivatization of peptides from Angiostrongylus costaricensis, a nematode with unknown genome | pt_BR |
Type | Article | pt_BR |
DOI | 10.1002/jms.1214 | |
Abstract | Matrix-assisted laser desorption ionization (MALDI), Peptide Mass Fingerprinting (PMF) and MALDI-MS/MS ion search (using MASCOT) have become the preferred methods for high-throughput identification of proteins. Unfortunately, PMF can be ambiguous, mainly when the genome of the organism under investigation is unknown and the quality of spectra generated is poor and does not allow confident identification. The post-source decay (PSD) fragmentation of singly charged tryptic peptide ions generated by MALDI-TOF/TOF typically results in low fragmentation efficiency and/or complex spectra, including backbone fragmentation ions (series b and y), internal fragmentation etc. Interpreting these data either manually and/or using de novo sequencing software can frequently be a challenge. To overcome this limitation when studying the proteome of adult Angiostrongylus costaricensis, a nematode with unknown genome, we have used chemical N-terminal derivatization of the tryptic peptides with 4-sulfophenyl isothiocyanate (SPITC) prior to MALDI-TOF/TOF MS. This methodology has recently been reported to enhance the quality of MALDI-TOF/TOF-PSD data, allowing the obtainment of complete sequence of most of the peptides and thus facilitating de novo peptide sequencing. Our approach, consisting of SPITC derivatization along with manual spectra interpretation and Blast analysis, was able to positively identify 76% of analyzed samples, whereas MASCOT analysis of derivatized samples, MASCOT analysis of nonderivatized samples and PMF of nonderivatized samples yielded only 35, 41 and 12% positive identifications, respectively. Moreover, de novo sequencing of SPITC modified peptides resulted in protein sequences not available in NCBInr database paving the way to the discovery of new protein molecules. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Patologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | SPITC | pt_BR |
Subject | MALDI TOF/TOF | pt_BR |
Subject | PSD | pt_BR |
Subject | de novo sequencing | pt_BR |
Subject | Angiostrongylus costaricensis | pt_BR |
DeCS | Sequência de Aminoácidos | pt_BR |
DeCS | Angiostrongylus, química | pt_BR |
DeCS | Proteínas de Helminto | pt_BR |
DeCS | Dados de Sequência Molecular | pt_BR |
DeCS | Mapeamento de Peptídeos | pt_BR |