Author | Silva, Daniela Carla Medeiros | |
Author | Moreira-Silva, Eduardo Augusto dos Santos | |
Author | Gomes, Juliana de Assis Silva | |
Author | Fonseca, Flávio Guimarães da | |
Author | Oliveira, Rodrigo Correa | |
Access date | 2023-07-04T17:13:56Z | |
Available date | 2023-07-04T17:13:56Z | |
Document date | 2013 | |
Citation | SILVA, Daniela Carla Medeiros et al. CD4 and CD8 T cells participate in the immune memory response against Vaccinia virus after a previous natural infection. Results in Immunology, v. 3, p. 104-113, 2013. | en_US |
ISSN | 2211-2839 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/59404 | |
Language | eng | en_US |
Publisher | Elsevier | en_US |
Rights | restricted access | en_US |
Title | CD4 and CD8 T cells participate in the immune memory response against Vaccinia virus after aprevious natural infection | en_US |
Type | Article | en_US |
DOI | 10.1016/j.rinim.2013.10.002 | |
Abstract | The present study evaluates the immune response of memory CD4+ and CD8+ T cells from patients following a natural Vaccinia virus (VACV) infection. A total of 42 individuals were involved in the study being: 22 previously infected individuals (vaccinated or not against smallpox) and 20 non-infected individuals (vaccinated or not). A short-term in vitro stimulation with UV-inactivated VACV of whole blood cells was performed. Our study showed that previously infected individuals have a lower percentage of CD4+ T cells expressing lymph-node homing receptors (CD4+CD62L+CCR7+) and higher percentage of memory CD4+ T cells subsets (CD4+CD45ROHigh) when compared with non-infected subjects, after in vitro viral stimulation. We also showed that infected individuals presented higher percentages of CD4+ and CD8+ memory T lymphocytes expressing IFN-γ when compared to non-infected individuals. We verified that the percentage of CD4+ and CD8+ T memory cells expressing TNF-α was higher in infected and non-infected vaccinated subjects when compared with non-infected unvaccinated individual. We also observed that previously infected individuals have higher percentages of CD8+ T cells expressing lymph-node homing receptors (CCR7+ and CD62L+) and that the memory T cells expressing IFN-γ and TNF-α were at higher percentages in the whole blood cells from infected and non-infected vaccinated individuals, when compared to unvaccinated non-infected subjects. Thus, our findings suggest that CD4+ and CD8+ T cells are involved in the immune memory response against Vaccinia virus natural infection. | en_US |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil / Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil / Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil / Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil. | en_US |
Subject | Vaccinia virus | en_US |
Subject | Poxviridae infections | en_US |
Subject | Infection | en_US |
Subject | Zoonoses | en_US |
Subject | Smallpox vaccine | en_US |
Embargo date | 2030-12-31 | |