Author | Alves, Fernanda Moreira | |
Author | Lisboa, Cristiane Varella | |
Author | Dario, Maria Augusta | |
Author | Novaes, Roberto Leonan Morim | |
Author | Tiepolo, Liliani Marilia | |
Author | Moratelli, Ricardo | |
Author | Jansen, Ana Maria | |
Access date | 2023-06-26T16:42:57Z | |
Available date | 2023-06-26T16:42:57Z | |
Document date | 2023 | |
Citation | ALVES, Fernanda Moreira et al. Old Methods, New Insights: Reviewing Concepts on the Ecology of Trypanosomatids and Bodo sp. by Improving Conventional Diagnostic Tools. Pathogens, v. 12, 71, p. 1 - 14, Jan. 2023. | en_US |
ISSN | 2076-0817 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/59178 | |
Language | eng | en_US |
Publisher | MDPI | en_US |
Rights | open access | |
Subject in Portuguese | Trypanosoma | en_US |
Subject in Portuguese | Bodo | en_US |
Subject in Portuguese | Ferramentas de diagnóstico | en_US |
Subject in Portuguese | Hemocultura | en_US |
Subject in Portuguese | Coágulo sanguíneo | en_US |
Subject in Portuguese | Seleção | en_US |
Subject in Portuguese | Infecções mistas | en_US |
Subject in Portuguese | Morcegos | en_US |
Title | Old Methods, New Insights: Reviewing Concepts on the Ecology of Trypanosomatids and Bodo sp. by Improving Conventional Diagnostic Tools | en_US |
Type | Article | |
DOI | 10.3390/ pathogens12010071 | |
Abstract | Mixed infections by different Trypanosoma species or genotypes are a common and puzzling
phenomenon. Therefore, it is critical to refine the diagnostic techniques and to understand to what
extent these methods detect trypanosomes. We aimed to develop an accessible strategy to enhance
the sensitivity of the hemoculture, as well as to understand the limitations of the hemoculture and
the blood clot as a source of parasitic DNA.We investigated trypanosomatid infections in 472 bats
by molecular characterization (18S rDNA gene) of the DNA obtained from the blood clot and,
innovatively, from three hemoculture sample types: the amplified flagellates (“isolate”), the pellet of
the culture harvested in its very initial growth stage (“first aliquot”), and the pellet of non-grown
cultures with failure of amplification (“sediment”). We compared (a) the characterization of the
flagellates obtained by first aliquots and isolates; and (b) the performance of the hemoculture and
blood clot for trypanosomatid detection. We observed: (i) a putative new species of Bodo in Artibeus
lituratus; (ii) the potential of Trypanosoma cruzi selection in the hemoculture; (iii) that the first aliquots
and sediments overcome the selective pressure of the hemoculture; and (iv) that the blood clot
technique performs better than the hemoculture. However, combining these methods enhances the
detection of single and mixed infections. | en_US |
Affilliation | Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Fiocruz Mata Atlântica. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Universidade Federal do Paraná. Coastal Campus. Laboratório de Análise e Monitoramento da Mata Atlântica. Matinhos, PR, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Fiocruz Mata Atlântica. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Biologia de Tripanossomatídeos. Rio de Janeiro, RJ, Brasil. | en_US |
Subject | Trypanosoma | en_US |
Subject | Bodo | en_US |
Subject | Diagnostic tools | en_US |
Subject | Hemoculture | en_US |
Subject | Blood clot | en_US |
Subject | Selection | en_US |
Subject | Mixed infections | en_US |
Subject | Bats | en_US |