Author | Marin, Adriana A. | |
Author | Juillard, Annette | |
Author | Katzin, Alejandro M. | |
Author | Carvalho, Leonardo J. M. | |
Author | Grau, Georges E. R. | |
Access date | 2023-06-12T11:34:51Z | |
Available date | 2023-06-12T11:34:51Z | |
Document date | 2023 | |
Citation | MARIN, Adriana A. et al. Perillyl alcohol modulates activation, permeability and integrity of human brain endothelial cells induced by Plasmodium falciparum. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 118, e230033, p. 1 - 9, 2023. | en_US |
ISSN | 0074-0206 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/58988 | |
Language | eng | en_US |
Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz | en_US |
Rights | open access | |
Subject in Portuguese | Terpenos | en_US |
Subject in Portuguese | Células endoteliais do cérebro humano | en_US |
Subject in Portuguese | P. falciparum | en_US |
Subject in Portuguese | Malária cerebral | en_US |
Subject in Portuguese | Moléculas de adesão celular | en_US |
Title | Perillyl alcohol modulates activation, permeability and integrity of human brain endothelial cells induced by Plasmodium falciparum | en_US |
Type | Article | |
DOI | 10.1590/0074-02760230033 | |
Abstract | characterised by the sequestration of parasitised red blood cells (pRBCs) in brain microvessels. Previous studies have shown
that some terpenes, such as perillyl alcohol (POH), exhibit a marked efficacy in preventing cerebrovascular inflammation,
breakdown of the brain-blood barrier (BBB) and brain leucocyte accumulation in experimental CM models.
OBJECTIVE To analyse the effects of POH on the endothelium using human brain endothelial cell (HBEC) monolayers cocultured
with pRBCs.
METHODOLOGY The loss of tight junction proteins (TJPs) and features of endothelial activation, such as ICAM-1 and VCAM-1
expression were evaluated by quantitative immunofluorescence. Microvesicle (MV) release by HBEC upon stimulation by P.
falciparum was evaluated by flow cytometry. Finally, the capacity of POH to revert P. falciparum-induced HBEC monolayer
permeability was examined by monitoring trans-endothelial electrical resistance (TEER).
FINDINGS POH significantly prevented pRBCs-induced endothelial adhesion molecule (ICAM-1, VCAM-1) upregulation and
MV release by HBEC, improved their trans-endothelial resistance, and restored their distribution of TJPs such as VE-cadherin,
Occludin, and JAM-A.
CONCLUSIONS POH is a potent monoterpene that is efficient in preventing P. falciparum-pRBCs-induced changes in HBEC,
namely their activation, increased permeability and alterations of integrity, all parameters of relevance to CM pathogenesis. | en_US |
Affilliation | Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Parasitologia, São Paulo, SP, Brasil / Universit.y of Sydney, Department of Pathology, Vascular Immunology Unit, Sydney Medical School, New South Wales, Australia. | en_US |
Affilliation | Universit.y of Sydney, Department of Pathology, Vascular Immunology Unit, Sydney Medical School, New South Wales, Australia. | en_US |
Affilliation | Universidade de São Paulo, Instituto de Ciências Biomédicas, Departamento de Parasitologia, São Paulo, SP, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Universit.y of Sydney, Department of Pathology, Vascular Immunology Unit, Sydney Medical School, New South Wales, Australia. | en_US |
Subject | Terpenes | en_US |
Subject | Human brain endothelial cells | en_US |
Subject | P. falciparum | en_US |
Subject | Cerebral malaria | en_US |
Subject | Cell adhesion molecules | en_US |