Author | Oliveira, Luciana Bonome Zeminian de | |
Author | Coletta, Amanda Manoel Della | |
Author | Gardizani, Taiane Priscila | |
Author | Garces, Hans Garcia | |
Author | Bagagli, Eduardo | |
Author | Trilles, Luciana | |
Author | Barrozo, Ligia Vizeu | |
Author | Marques, Sílvio de Alencar | |
Author | De Faveri, Julio | |
Author | Dias-Melicio, Luciane Alarcão | |
Access date | 2023-06-08T00:48:39Z | |
Available date | 2023-06-08T00:48:39Z | |
Document date | 2023 | |
Citation | OLIVEIRA, Luciana Bonome Zeminian de et al. Molecular Phylogenetic Analysis of Paracoccidioides Species Complex Present in Paracoccidioidomycosis Patient Tissue Samples. Microorganisms, v. 11, n. 3, p. 1-13, Feb. 2023. | en_US |
ISSN | 2076-2607 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/58978 | |
Sponsorship | This work was supported by research funding from São Paulo Research Foundation (FAPESP) through the Ph.D. fellowship (FAPESP 2013/24877-9). The funders had no role in study design, data collection, and analysis, the decision to publish, or preparation of the manuscript. | en_US |
Language | eng | en_US |
Publisher | MDPI | en_US |
Rights | open access | en_US |
Title | Molecular Phylogenetic Analysis of Paracoccidioides Species Complex Present in Paracoccidioidomycosis Patient Tissue Samples | en_US |
Type | Article | en_US |
DOI | 10.3390/microorganisms11030562 | |
Abstract | Paracoccidioidomycosis (PCM) is the main and most prevalent systemic mycosis in Latin America, that until recently, it was believed to be caused only by Paracoccidioides brasiliensis (P. brasiliensis). In 2006, researchers described three cryptic species: S1, PS2, PS3, and later, another one, PS4. In 2009, Paracoccidioides lutzii (Pb01-like) was described, and in 2017, a new nomenclature was proposed for the different agents: P. brasiliensis (S1), P. americana (PS2), P. restrepiensis (PS3), and P. venezuelensis (PS4). These species are not uniformly distributed throughout Latin America and, knowing that more than one cryptic species could coexist in some regions, we aimed to identify those species in patients' biopsy samples for a better understanding of the distribution and occurrence of these recently described species in Botucatu region. The Hospital of Medical School of Botucatu-UNESP, which is a PCM study pole, is located in São Paulo State mid-west region and is classified as a PCM endemic area. Genotyping analyses of clinical specimens from these patients that have been diagnosed and treated in our Hospital could favor a possible correlation between genetic groups and mycological and clinical characteristics. For this, molecular techniques to differentiate Paracoccidioides species in these biopsies, such as DNA extraction, PCR, and sequencing of three target genes (ITS, CHS2, and ARF) were conducted. All the sequences were analyzed at BLAST to testify the presence of P. brasiliensis. The phylogenetic trees were constructed using Mega 7.0 software and showed that 100% of our positive samples were from S1 cryptic species, therefore P. brasiliensis. This is important data, demonstrating the predominance of this species in the São Paulo State region. | en_US |
Affilliation | São Paulo State University. Medical School of Botucatu. UNIPEX-Experimental Research Unity-Sector 5. Laboratory of Immunopathology and Infectious Agents-LIAI. Botucatu, SP, Brazil. | en_US |
Affilliation | São Paulo State University. Medical School of Botucatu. UNIPEX-Experimental Research Unity-Sector 5. Laboratory of Immunopathology and Infectious Agents-LIAI. Botucatu, SP, Brazil. | en_US |
Affilliation | São Paulo State University. Medical School of Botucatu. UNIPEX-Experimental Research Unity-Sector 5. Laboratory of Immunopathology and Infectious Agents-LIAI. Botucatu, SP, Brazil. | en_US |
Affilliation | São Paulo State University. Institute of Biosciences. Department of Chemical and Biological Sciences. Botucatu, SP, Brazil. | en_US |
Affilliation | São Paulo State University. Institute of Biosciences. Department of Chemical and Biological Sciences. Botucatu, SP, Brazil. | en_US |
Affilliation | Oswaldo Cruz Foundation. Evandro Chagas National Institute of Infectious Disease. Rio de Janeiro, RJ, Brazil. | en_US |
Affilliation | University of São Paulo. Literature and Human Sciences. Department of Geography, School of Philosophy. São Paulo, SP, Brazil. | en_US |
Affilliation | São Paulo State University. Department of Dermatology, Medical School of Botucatu. Botucatu, SP, Brazil. | en_US |
Affilliation | São Paulo State University. Medical School of Botucatu. Department of Pathology. Botucatu, SP, Brazil. | en_US |
Affilliation | São Paulo State University. Medical School of Botucatu. UNIPEX-Experimental Research Unity-Sector 5. Laboratory of Immunopathology and Infectious Agents-LIAI. Botucatu, SP, Brazil / São Paulo State University. Medical School of Botucatu. Department of Pathology. Botucatu, SP, Brazil. | en_US |
Subject | Paracoccidioides brasiliensis | en_US |
Subject | Paracoccidioides species complex | en_US |
Subject | Molecular Biology | en_US |
Subject | Molecular phylogenetic analysis | en_US |
Subject | Paracoccidiodomycosis | en_US |
e-ISSN | 2076-2607 | |