Author | Mendes-Aguiar, Carolina de O. | |
Author | Kitahara-Oliveira, Milene Yoko | |
Author | Almeida, Ana Cristina Oliveira de | |
Author | Pereira-Oliveira, Marcia | |
Author | Oliveira Neto, Manoel Paes de | |
Author | Pirmez, Claude | |
Author | Sampaio, Elizabeth Pereira | |
Author | Gomes-Silva, Adriano | |
Author | Da-Cruz, Alda Maria | |
Access date | 2023-06-07T23:27:24Z | |
Available date | 2023-06-07T23:27:24Z | |
Document date | 2023 | |
Citation | MENDES-AGUIAR, Carolina de O. et al. DC-SIGN receptor is expressed by cells from cutaneous leishmaniasis lesions and differentially binds to Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis promastigotes. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 18, p. 1-7, Feb. 2023. | en_US |
ISSN | 0074-0276 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/58973 | |
Sponsorship | This work was supported by IOC/FIOCRUZ internal funds and FAPERJ (E-26/102.457/2010). COM-A and pos-doctor are sponsored by FAPERJ/CAPES, Brazil; MYKO is a doctorate student sponsored by IOC/FIOCRUZ; AMD-C was sponsored by CNPq and FAPERJ research fellows. | en_US |
Language | eng | en_US |
Publisher | Fiocruz/IOC | en_US |
Previous version | https://www.arca.fiocruz.br/handle/icict/57316 | en_US |
Rights | open access | |
Title | DC-SIGN receptor is expressed by cells from cutaneous leishmaniasis lesions and differentially binds to Leishmania (Viannia) braziliensis and L. (Leishmania) amazonensis promastigotes | en_US |
Type | Article | |
DOI | 10.1590/0074-02760220044 | |
Abstract | Background: Dendritic cells (DCs) specific intercellular adhesion molecule (ICAM)-3-grabbing non integrin receptor (DC-SIGN) binds to subgenera Leishmania promastigotes mediating its interaction with DC and neutrophils, potentially influencing the infection outcome. Objectives: In this work, we investigated whether DC-SIGN receptor is expressed in cells from cutaneous leishmaniasis (CL) lesions as well as the in vitro binding pattern of Leishmania (Viannia) braziliensis (Lb) and L. (L.) amazonensis (La) promastigotes. Methods: DC-SIGN receptor was labeled by immunohistochemistry in cryopreserved CL tissue fragments. In vitro binding assay with CFSE-labeled Lb or La promastigotes and RAJI-transfecting cells expressing DC-SIGN (DC-SIGNPOS) or mock-transfected (DC-SIGNNEG) were monitored by flow cytometry at 2 h, 24 h and 48 h in co-culture. Results: In CL lesion infiltrate, DC-SIGNPOS cells were present in the dermis and near the epidermis. Both Lb and La bind to DC-SIGNPOS cells, while binding to DC-SIGNNEG was low. La showed precocious and higher affinity to DC-SIGNhi population than to DC-SIGNlow, while Lb binding was similar in these populations. Conclusion: Our results demonstrate that DC-SIGN receptor is present in L. braziliensis CL lesions and interact with Lb promastigotes. Moreover, the differences in the binding pattern to Lb and La suggest DC-SIGN can influence in a difference way the intake of the parasites at the first hours after Leishmania infection. These results raise the hypothesis that DC-SIGN receptor could participate in the immunopathogenesis of American tegumentary leishmaniasis accounting for the differences in the outcome of the Leishmania spp. infection. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio Grande do Norte. Instituto de Medicina Tropical do Rio Grande do Norte. Natal, RN, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Disciplina de Parasitologia, DMIP. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Micobacterioses. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | National Institute of Allergy and Infectious Disease. Laboratory of Clinical Immunology and Microbiology. Bethesda, USA / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Micobacterioses. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Disciplina de Parasitologia, DMIP. Rio de Janeiro, RJ, Brasil. | en_US |
Subject | DC-SIGN receptor | en_US |
Subject | Cutaneous leishmaniasis | en_US |
Subject | Inflammatory infiltrate | en_US |
Subject | Leishmania (Viannia) braziliensis | en_US |
Subject | Leishmania (Leishmania) amazonensis | en_US |
e-ISSN | 1678-8060 | |