Author | Gomes, Luciana Inácia | |
Author | Marques, Letícia Helena dos Santos | |
Author | Enk, Martin Johannes | |
Author | Coelho, Paulo Marcos Zech | |
Author | Rabello, Ana Lucia Teles | |
Access date | 2023-05-29T13:53:33Z | |
Available date | 2023-05-29T13:53:33Z | |
Document date | 2009 | |
Citation | GOMES, Luciana Inácia et al. Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples. Mem Inst Oswaldo Cruz, v.104, n. 8, p. 1194-1196, 2009. doi: 10.1590/s0074-02762009000800021. | en_US |
ISSN | 0074-0276 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/58794 | |
Language | eng | en_US |
Publisher | Ministério da Saúde | en_US |
Rights | open access | en_US |
Title | Further evaluation of an updated PCR assay for the detection of Schistosoma mansoni DNA in human stool samples | en_US |
Type | Article | en_US |
DOI | https://doi.org/10.1590/s0074-02762009000800021 | |
Abstract | A previously reported sensitive PCR assay for the detection of Schistosoma mansoni DNA was updated and evaluated. Changes in the DNA extraction method, including the use of a worldwide available commercial kit and the inclusion of additional quality control measures, increased the robustness of the test, as confirmed by the analysis of 67 faecal samples from an endemic area in Brazil. The PCR assay is at hand as a proven, reliable diagnostic test for the control of schistosomiasis in specific settings | en_US |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil | en_US |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil | en_US |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil | en_US |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil | en_US |
Subject | schistosomiasis | en_US |
Subject | molecular diagnosis | en_US |
Subject | faecal samples | en_US |
Subject | polymerase chain reaction | en_US |