Author | Magalhães, Fernanda do Carmo | |
Author | Resende, Samira Diniz | |
Author | Senra, Carolina | |
Author | Graeff-Teixeira, Carlos | |
Author | Enk, Martin Johannes | |
Author | Coelho, Paulo Marcos Zech | |
Author | Oliveira, Edward Jose de | |
Author | Negrão-Corrêa, Deborah Aparecida | |
Author | Geiger, Stefan Michael | |
Author | Carneiro, Mariângela | |
Access date | 2023-05-19T17:11:30Z | |
Available date | 2023-05-19T17:11:30Z | |
Document date | 2020 | |
Citation | MAGALHÃES, Fernanda do Carmo et al. Accuracy of real-time polymerase chain reaction to detect Schistosoma mansoni – infected individuals from an endemic area with low parasite loads. Parasitology, v. 147, n. 10, p. 1140-1148, Jun. 2020. | en_US |
ISSN | 0031-1820 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/58521 | |
Language | eng | en_US |
Publisher | Cambridge University Press | en_US |
Rights | restricted access | en_US |
Title | Accuracy of real-time polymerase chain reaction to detectSchistosoma mansoni - infected individuals from an endemic area with low parasite loads | en_US |
Type | Article | en_US |
DOI | 10.1017/S003118202000089X | |
Abstract | Due to the efforts to control schistosomiasis transmission in tropical countries, a large proportion of individuals from endemic areas present low parasite loads, which hinders diagnosis of intestinal schistosomiasis by the Kato-Katz (KK) method. Therefore, the development of more sensitive diagnostic methods is essential for efficient control measures. The aim was to evaluate the accuracy of a real-time polymerase chain reaction (RT-PCR) to detectSchistosoma mansoniDNA in fecal samples of individuals with low parasite loads. A cross-sectional population-based study was conducted in a rural community (n= 257) in Brazil. POC-CCA(R)was performed in urine and feces were used for RT-PCR. In addition, fecal exams were completed by 18 KK slides, saline gradient and Helmintex techniques. The combined results of the three parasitological tests detected schistosome eggs in 118 participants (45.9%) and composed the consolidated reference standard (CRS). By RT-PCR, 117 out of 215 tested samples were positive, showing 91.4% sensitivity, 80.2% specificity and good concordance with the CRS (kappa = 0.71). RT-PCR identified 86.9% of the individuals eliminating less than 12 eggs/g of feces, demonstrating much better performance than POC-CCA(R)(50.8%). Our results showed that RT-PCR is a valuable alternative for the diagnosis of intestinal schistosomiasis in individuals with very low parasite loads. | en_US |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Pesquisa Clínica e Políticas Públicas em Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Universidade Federal do Espírito Santo. Centro de Ciências da Saúde. Núcleo de Doenças Infecciosas. Vitória, ES, Brazil. | en_US |
Affilliation | Ministério da Saúde. Instituto Evandro Chagas. Secretaria de Vigilância em Saúde. Belém, PA, Brazil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Diagnóstico e Terapia de Doenças Infecciosas e Oncológicas. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Pesquisa Clínica e Políticas Públicas em Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil. | en_US |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brazil. | en_US |
Subject | Diagnosis | en_US |
Subject | Low parasite load | en_US |
Subject | RT-PCR | en_US |
Subject | Schistosomiasis mansoni | en_US |
Embargo date | 2099-12-31 | |