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https://www.arca.fiocruz.br/handle/icict/57892
CRISPR-ASSISTED TEST FOR SCHISTOSOMA HAEMATOBIUM
Author
Affilliation
London Centre for Nanotechnology. University College London. London, WC1H 0AH, UK/Division of Medicine. University College London. London, WC1E 6BT, UK.
Wolfson Wellcome Biomedical Laboratories, Department of Science, Natural History Museum, Cromwell Road, London, SW7 5BD, UK/René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil/London Centre for Neglected Tropical Disease Research. London, W21 PG, UK.
London Centre for Nanotechnology. University College London. London, WC1H 0AH, UK.
Wolfson Wellcome Biomedical Laboratories, Department of Science, Natural History Museum, Cromwell Road, London, SW7 5BD, UK/London Centre for Neglected Tropical Disease Research. London, W21 PG, UK.
Wolfson Wellcome Biomedical Laboratories. Department of Science. Natural History Museum. Cromwell Road, London, SW7 5BD, UK/London Centre for Neglected Tropical Disease Research. London, W21 PG, UK.
London Centre for Nanotechnology. University College London. London, WC1H 0AH, UK/Division of Medicine. University College London. London, WC1E 6BT, UK.
Wolfson Wellcome Biomedical Laboratories, Department of Science, Natural History Museum, Cromwell Road, London, SW7 5BD, UK/René Rachou Institute. Oswaldo Cruz Foundation. Belo Horizonte, MG, Brazil/London Centre for Neglected Tropical Disease Research. London, W21 PG, UK.
London Centre for Nanotechnology. University College London. London, WC1H 0AH, UK.
Wolfson Wellcome Biomedical Laboratories, Department of Science, Natural History Museum, Cromwell Road, London, SW7 5BD, UK/London Centre for Neglected Tropical Disease Research. London, W21 PG, UK.
Wolfson Wellcome Biomedical Laboratories. Department of Science. Natural History Museum. Cromwell Road, London, SW7 5BD, UK/London Centre for Neglected Tropical Disease Research. London, W21 PG, UK.
London Centre for Nanotechnology. University College London. London, WC1H 0AH, UK/Division of Medicine. University College London. London, WC1E 6BT, UK.
Abstract
Schistosomiasis is a major neglected tropical disease targeted for elimination as a public health issue by 2030, however there is an urgent need for more sensitive and specific diagnostic tests suitable to resource-limited settings. Here we developed CATSH, a CRISPR-assisted diagnostic test for Schistosoma haematobium, utilising recombinase polymerase amplification, Cas12a-targeted cleavage and portable real-time fluorescence detection. CATSH showed high analytical sensitivity, consistent detection of a single parasitic egg and specificity for urogenital Schistosoma species. Thanks to a novel CRISPR-compatible sample preparation developed using simulated urine samples containing parasitic eggs, CATSH had a sample-to-result within 2 h. The components of CATSH can be lyophilised, reducing cold chain dependence and widening access to lower and middle-income countries. This work presents a new application of CRISPR diagnostics for highly sensitive and specific detection of parasitic pathogens in remote areas and could have a significant impact on the elimination of neglected tropical diseases.
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