Author | Martins, Paulo Henrique Rosa | |
Author | Rabinovitch, Leon | |
Author | Orem, Juliana Capela de | |
Author | Silva, Waldeyr Mendes C. | |
Author | Mesquita, Felipe de Araujo | |
Author | Magalhães, Maria Ines Andre de | |
Author | Cavalcante, Danilo de Andrade | |
Author | Vivoni, Adriana Marcos | |
Author | Oliveira, Edmar Justo de | |
Author | Lima, Vera Cristina Pessoa de | |
Author | Brito, Josiane Teixeira | |
Author | Souza, Marlene Teixeira de | |
Access date | 2023-04-03T11:49:41Z | |
Available date | 2023-04-03T11:49:41Z | |
Document date | 2023 | |
Citation | MARTINS, Paulo Henrique Rosa et al. Biochemical, physiological, and molecular characterisation of a large collection of aerobic endospore-forming bacteria isolated from Brazilian soils. Neotropical Biology and Conservation, v.18, n.1, p. 53-72, Feb. 2023. | en_US |
ISSN | 2236-3777 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/57653 | |
Language | eng | en_US |
Publisher | Pensoft | en_US |
Rights | open access | |
Subject in Portuguese | Bacilos | en_US |
Subject in Portuguese | Identificação bacteriana | en_US |
Subject in Portuguese | Metabolismo bacteriano | en_US |
Subject in Portuguese | Firmicutes | en_US |
Subject in Portuguese | Fenotipagem | en_US |
Subject in Portuguese | Taxonomia | en_US |
Subject in Portuguese | Endosporulação | en_US |
Title | Biochemical, physiological, and molecular characterisation of a large collection of aerobic endospore-forming bacteria isolated from Brazilian soils | en_US |
Type | Article | |
DOI | 10.3897/neotropical.18.e86548 | |
Abstract | The aerobic endospore-forming bacteria (AEFB) comprise species of Bacillus and related genera and have long been regarded as prominent constituents of the soil bacterial community. The wide diversity of AEFB renders appropriate categorisation and generalisations a challenging task. We previously isolated 312 AEFB strains from Brazilian soils that we designated SDF (Solo do Distrito Federal) strains. To better understand the SDF diversity and explore their biotechnological potential, we addressed the biochemical and physiological profiles of these 312 environmental strains by performing 30 tests in this work. Of these, the 16S rRNA gene sequences segregated 238 SDF strains into four genera in the family Bacillaceae and two in the Paenibacillaceae. Bacillus spp. were the most prevalent, followed by species of Paenibacillus. We summarised the phenotypic test relationships among selected SDF strains using a Pearson correlation-based clustering represented in heatmaps. In practice, biochemical and physiological profiles are often less discriminatory than molecular data and may be unstable because of the loss of traits. Although these test reactions are not
universally positive or negative within species, they may define biotypes and be efficient strain markers,
enhancing the accuracy of unknown sample identification. It can also help select the most representative
phenotypes of samples. Along with the other phenotypic and genotypic data, the present results are of great
importance for the robust classification of the SDF strains within the scope of the polyphasic approach. | en_US |
Affilliation | Universidade de Brasília. Instituto de Ciências Biológicas. Departamento de Biologia Celular. Brasília, DF, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Universidade de Brasília. Instituto de Ciências Biológicas. Departamento de Biologia Celular. Brasília, DF, Brasil. | en_US |
Affilliation | Instituto Federal de Goias. Formosa, GO, Brasil. | en_US |
Affilliation | Universidade de Brasília. Instituto de Ciências Biológicas. Departamento de Biologia Celular. Brasília, DF, Brasil. | en_US |
Affilliation | Universidade de Brasília. Instituto de Ciências Biológicas. Departamento de Biologia Celular. Brasília, DF, Brasil. | en_US |
Affilliation | Universidade de Brasília. Instituto de Ciências Biológicas. Departamento de Biologia Celular. Brasília, DF, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia Bacteriana. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Universidade de Brasília. Instituto de Ciências Biológicas. Departamento de Biologia Celular. Brasília, DF, Brasil. | en_US |
Subject | Bacillales | en_US |
Subject | Bacterial identification | en_US |
Subject | Bacterial metabolism | en_US |
Subject | Firmicutes | en_US |
Subject | Phenotyping | en_US |
Subject | Taxonomy | en_US |
Subject | Endosporulation | en_US |