Please use this identifier to cite or link to this item:
https://www.arca.fiocruz.br/handle/icict/57617
Type
ArticleCopyright
Open access
Collections
- IOC - Artigos de Periódicos [12494]
Metadata
Show full item record
ASSESSMENT OF VIRUS CONCENTRATION METHODS FOR DETECTING SARS‑COV‑2 IN WASTEWATER
Ultracentrifugação
Floculação de leite desnatado
Eficiência de recuperação
Limite de detecção
Vírus sincicial respiratório bovino - BRSV
SARS-CoV-2
BRSV
Skimmed-milk flocculation
Recovery efficiency
Limit of detection
BRSV- bovine respiratory syncytial virus
Author
Affilliation
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Departamento de Saneamento e Saúde Ambiental. Rio de Janeiro, RJ, Brasil. RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro. Rio de Janeiro, Nilópolis, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro. Rio de Janeiro, Nilópolis, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
Abstract
Abstract
Wastewater-based epidemiology has been described as a valuable tool for monitoring the spread of severe acute respiratory
syndrome coronavirus 2 (SARS-CoV-2) in a community. However, there is no consensus on the best concentration
method to allow reliable detection of SARS-CoV-2 in this matrix, considering different laboratory facilities. This study
compares two viral concentration methods, ultracentrifugation (ULT) and skimmed-milk flocculation (SMF), for detecting
SARS-CoV-2 in wastewater samples. The analytical sensitivity (limits of detection and quantification [LoD/LoQ]) of both
methods was evaluated using a bovine respiratory syncytial virus (BRSV) as a surrogate. Three different approaches were
conducted to establish LoD of each method based on the assays on the standard curve (ALoDsc), on the dilution of internal
control (ALoDiC), and the processing steps (PLoD). For PLoD, ULT method had the lowest value (1.86 × 103
genome copy/
microliter [GC/μL]) when compared to the SMF method (1.26 × 107
GC/μL). The LoQ determination showed a mean value
of 1.55 × 105
GC/μL and 3.56 × 108
GC/μL to ULT and SMF, respectively. The detection of SARSCoV-2 in naturally contaminated
wastewater revealed 100% (12/12) and 25% (3/12) of detection using ULT and SMF with quantification ranging
from 5.2 to 7.2 log10 genome copy/liter (GC/L) and 5.06 to 5.46 log10 GC/L, respectively. The detection success rate of
BRSV used as an internal control process was 100% (12/12) for ULT and 67% (8/12) for SMF, with an efficiency recovery
rate ranging from 12 to 38% and 0.1 to 5%, respectively. Our data consolidates the importance of assessing the methods
used; however, further analysis should be carried out to improve low-cost concentration methodologies, essential for use in
low-income and developing countries.
Keywords in Portuguese
SARS-CoV-2Ultracentrifugação
Floculação de leite desnatado
Eficiência de recuperação
Limite de detecção
Vírus sincicial respiratório bovino - BRSV
Keywords
UltracentrifugationSARS-CoV-2
BRSV
Skimmed-milk flocculation
Recovery efficiency
Limit of detection
BRSV- bovine respiratory syncytial virus
Share