Author | Silva, Mariana Barbalho Farias da | |
Author | Mota, Fabio Faria | |
Author | Jurelevicius, Diogo | |
Author | Azevedo, Vasco Ariston de Carvalho | |
Author | Costa, Mateus Matiuzzi da | |
Author | Góes Neto, Aristóteles | |
Author | Ramos, Rommel Thiago Jucá | |
Author | Castro Soares, Siomar de | |
Author | Rosado, Alexandre S. | |
Author | Seldin, Lucy | |
Access date | 2023-03-06T19:19:30Z | |
Available date | 2023-03-06T19:19:30Z | |
Document date | 2022 | |
Citation | SILVA, Mariana Barbalho Faria da et al. Genomic analyses of a novel bioemulsifier-producing Psychrobacillus strain isolated from soil of King George Island, Antarctica. Polar Biology, p. 1 - 27, 2023. | en_US |
ISSN | 0722-4060 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/57249 | |
Description | Eprint version - Post-print | en_US |
Language | eng | en_US |
Publisher | Springer Science and Business Media LLC | en_US |
Rights | open access | |
Subject in Portuguese | Psychrobacillus | en_US |
Subject in Portuguese | Genoma | en_US |
Subject in Portuguese | Antarctica | en_US |
Subject in Portuguese | Bioemulsificante | en_US |
Subject in Portuguese | Adaptação de baixa temperatura | en_US |
Title | Genomic analyses of a novel bioemulsifierproducing Psychrobacillus strain isolated from soil of King George Island, Antarctica | en_US |
Type | Preprint | en_US |
Abstract | Cold-adapted bacterial strains are potentially valuable for biotechnological applications
27 involving the production of cold-active enzymes and bioproducts important to various
28 industries. A psychrotolerant, aerobic, Gram-positive, endospore-forming,
29 bioemulsifier-producing strain, named Val9, was isolated from Vale Ulman soil
30 samples, King George Island, Antarctica and identified as a member of the genus
31 Psychrobacillus. To better characterize this novel strain, its whole genome was
32 sequenced revealing a size of 3,986,526 bp with a G+C content of 36.6 %, and 4,042
33 predicted coding DNA sequences (CDSs). Digital DNA-DNA hybridization (dDDH)
34 and average nucleotide identity (ANI) analyses between strain Val9 and the type strains
35 of the seven Psychrobacillus species revealed that the highest values were observed
36 with Psychrobacillus psychrodurans DSM11713T but below the conventional
37 thresholds of 70 % dDDH and 95 % ANI for bacterial species assignment, suggesting
38 that strain Val9 could represent a distinct species. As potential low-temperature
39 adaptation strategies, genes encoding cold-shock proteins, transporters for glycine
40 betaine, carnitine and choline, and enzymes acting against oxidative stress were found
41 in Val9 genome. DEAD-box RNA helicases, important for cold and oxidative tolerance,
42 and a two-component signal transduction system related to plasmatic membrane fluidity
43 as well as biotechnologically important CDSs, related to levan production, were
44 detected. The sacB gene encoding the enzyme levansucrase was exclusive for Val9 and
45 it was not found in the other Psychrobacillus type strains. Altogether, the comparative
46 genomic analyses presented here highlight important metabolic pathways and the
47 biotechnological potential of this novel strain. | en_US |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brasil. | en_US |
Affilliation | Universidade Federal do Vale do São Francisco. Petrolina, BA, Brasil. | en_US |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brasil. | en_US |
Affilliation | Universidade Federal do Pará. Instituto de Ciências Biológicas. Pará. Brasil. | en_US |
Affilliation | Universidade Federal do Triângulo Mineiro. Instituto de Ciências Biológicas e Naturais. Uberaba, MG, Brasil. | en_US |
Affilliation | KAUST, King 15 Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia. | en_US |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. | en_US |
Subject | Psychrobacillus | en_US |
Subject | Genome | en_US |
Subject | Antarctica | en_US |
Subject | Bioemulsifier | en_US |
Subject | Low-temperature adaptation | en_US |