Author | Teles, Amanda Mara | |
Author | Xavier, Gabriel Silva | |
Author | Moreira, Wendel Fragoso de Freitas | |
Author | Moragas-Tellis, Carla Junqueira | |
Author | Chagas, Maria do Socorro dos Santos | |
Author | Behrens, Maria Dutra | |
Author | Silva, Ana Lúcia Abreu | |
Author | Bezerra, Geusa Felipa de Barros | |
Author | Calabrese, Kátia da Silva | |
Author | Nascimento, Maria do Desterro Soares Brandão | |
Author | Souza, Fernando Almeida | |
Access date | 2023-01-14T18:33:08Z | |
Available date | 2023-01-14T18:33:08Z | |
Document date | 2022 | |
Citation | TELES, Amanda Mara et al. Talaromyces purpurogenus from a marine-polluted environment inhibits pro-inflammatory markers in LPS-stimulated RAW 264.7 cells and in carrageenan-induced paw edema. Journal of King Saud University – Science, n. 34, 102021, p. 1 - 9, Apr. 2022. | en_US |
ISSN | 2210-8319 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/56461 | |
Language | eng | en_US |
Publisher | Elsevier B.V. on behalf of King Saud University | en_US |
Rights | open access | |
Subject in Portuguese | Meroterpenóide | en_US |
Subject in Portuguese | Fungo | en_US |
Subject in Portuguese | Citocina | en_US |
Subject in Portuguese | Inflamação | en_US |
Subject in Portuguese | Óxido nítrico | en_US |
Subject in Portuguese | Mastócitos | en_US |
Title | Talaromyces purpurogenus from a marine-polluted environment inhibits pro-inflammatory markers in LPS-stimulated RAW 264.7 cells and in carrageenan-induced paw edema | en_US |
Type | Article | |
DOI | 10.1016/j.jksus.2022.102021 | |
Abstract | Objectives: Talaromyces purpurogenus is a fungus species widely found in the marine environment. When
in a hostile environment, it produces a series of secondary metabolites with important biotechnological
potential, such as meroterpenoids. In our previous report, we demonstrated that the culture broth ethyl
acetate extract from T. purpurogenus reduced the cellular infiltration of lymphoid organs and circulating
levels of TNF-a. To elucidate this anti-inflammatory effect, this work aimed to study T. purpurogenus isolated
from a marine-polluted environment regarding the chemical composition of its culture broth and
mycelial ethyl acetate extract (CBEAE and MEAE respectively), as well as its action on proinflammatory
markers in LPS-stimulated RAW 264.7 cells and carrageenan-induced mice paw edema.
Methods: Chemical characterization was performed by HPLC-DAD-UV. Cytotoxicity in vitro and endotoxin
quantification in extracts were preformed prior the experiments. In vitro inhibitory effect of T. purpurogenus
extracts were evaluated by cytokines and nitrite quantification in cell supernatant of RAW
264.7 cells. The effect of T. purpurogenus in murine acute inflammation model of carrageenan-induced
paw edema was verified by thickness kinetic and histology evaluation.
Results: The CBEAE demonstrated a richer chemical composition than MEAE, presenting nine meroterpenoid
compounds, while MEAE displayed only two meroterpenoid and one phenolic compound.
Cytotoxicity in RAW 264.7 macrophages was not observed at any concentration treatment for both
extracts. Endotoxin quantification also demonstrated absence of endotoxins in both extracts. At
500 lg/mL, the CBEAE and MEAE extracts inhibited nitrite (p = 0.0450 and p = 0.0497), TNF-a
(p = 0.0076 and p = 0.0001) and IL-1b (p = 0.0042 and p = 0.0031), but only CBEAE inhibited IL-6 levels
in LPS-stimulated cells (p = 0.0234). CBEAE, but not MEAE, also inhibited nitrite (p = 0.0353) and IL-1b | en_US |
Affilliation | Universidade Federal do Maranhão. Pós-Graduação em Saúde do Adulto, São Luis, MA, Brasil. | en_US |
Affilliation | Universidade Federal do Maranhão. Rede Nordeste de Biotecnologia. São Luis, MA, Brasil. | en_US |
Affilliation | Universidade Estadual do Maranhão. São Luís, MA, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos—Farmanguinhos. Laboratório de Produtos Naturais para Saúde Pública (LPNSP). Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos—Farmanguinhos. Laboratório de Produtos Naturais para Saúde Pública (LPNSP). Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto de Tecnologia em Fármacos—Farmanguinhos. Laboratório de Produtos Naturais para Saúde Pública (LPNSP). Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Universidade Estadual do Maranhão. São Luís, MA, Brasil. | en_US |
Affilliation | Universidade Federal do Maranhão. Pós-Graduação em Saúde do Adulto, São Luis, MA, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Universidade Federal do Maranhão. Pós-Graduação em Saúde do Adulto, São Luis, MA, Brasil / Universidade Federal do Maranhão. Rede Nordeste de Biotecnologia. São Luis, MA, Brasil. | en_US |
Affilliation | Universidade Estadual do Maranhão. São Luís, MA, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. | en_US |
Subject | Meroterpenoid | en_US |
Subject | Fungus | en_US |
Subject | Cytokine | en_US |
Subject | Inflammation | en_US |
Subject | Nitric oxide | en_US |
Subject | Mast cells | en_US |