Author | Ramos, Gisele da Costa | |
Author | Silva-Silva, João Victor | |
Author | Watanabe, Luciano Almeida | |
Author | Siqueira, José Edson de Sousa | |
Author | Souza, Fernando Almeida | |
Author | Calabrese, Kátia S. | |
Author | Marinho, Andrey Moacir do Rosario | |
Author | Marinho, Patrícia Santana Barbosa | |
Author | Oliveira, Aldo Sena de | |
Access date | 2023-01-13T16:55:48Z | |
Available date | 2023-01-13T16:55:48Z | |
Document date | 2022 | |
Citation | RAMOS, Gisele da Costa et al. Phomoxanthone A, Compound of Endophytic Fungi Paecilomyces sp. and Its Potential Antimicrobial and Antiparasitic. Antibiotics, v. 11, 1332, p. 1 - 13, Sept. 2022. | en_US |
ISSN | 2079-6382 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/56456 | |
Language | eng | en_US |
Publisher | MDPI | en_US |
Rights | open access | |
Subject in Portuguese | Bacillus subtilis | en_US |
Subject in Portuguese | Leishmania amazonensis | en_US |
Subject in Portuguese | Trypanosoma cruzi | en_US |
Subject in Portuguese | S-ribosilomocisteína liase | en_US |
Subject in Portuguese | Estudos computacionais | en_US |
Subject in Portuguese | Fomoxantona A | en_US |
Title | Phomoxanthone A, Compound of Endophytic Fungi Paecilomyces sp. and Its Potential Antimicrobial and Antiparasitic | en_US |
Type | Article | |
DOI | 10.3390/antibiotics11101332 | |
Abstract | Abstract: The present work reports the isolation and biological evaluation of three dimeric xanthones
from Paecilomyces sp. EJC01.1 isolated as endophytic from Schnella splendens, a typical plant of
the Amazon. The compounds phomoxanthone A (1), phomoxanthone B (2) and dicerandrol B (3)
were isolated by chromatographic procedures and identified by spectroscopic methods of 1D and
2D NMR and MS. The extracts and compound 1 showed antimicrobial activities against Bacillus
subtilis, Escherichia coli, Staphylococcus aureus, Salmonella typhimurium and Pseudomonas aeruginosa.
The compound phomoxanthone A (1) showed greater inhibitory activity against B. subtilis (MIC of
7.81 g mL1); in addition, it also pronounced inhibitory effect against promastigote forms of Leishmania
amazonensis (IC50 of 16.38 1.079 g mL1) and epimastigote forms Trypanosoma cruzi (IC50 of
28.61 1.071 g mL1). To provide more information about the antibacterial activity of compound 1,
an unprecedented molecular docking study was performed using S-ribosyl-homocysteine lyase (LuxS)
(PDB ID 2FQO), which showed a possible interaction of phomoxanthone A with two of the residues
(His58 and Cys126) that are fundamental for the catalysis mechanism in B. subtilis, which may be
associated with the higher activity, when compared to other bacteria, observed in experimental
studies. Additionally, quantum studies (DFT) were performed, for which a low gap value (5.982 eV)
was observed, which corroborates the reactivity of phomoxanthone A. Thus, phomoxanthone A can
be a good agent against pathogenic bacteria. | en_US |
Affilliation | Universidade Federal do Pará. Programa de Pós-Graduação em Química. Belém, PA, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil / Universidade de São Paulo. Instituto de Física de São Carlos. Laboratório de Química Medicinal e Computacional. São Carlos, SP, Brasil. | en_US |
Affilliation | Universidade Federal do Pará. Programa de Pós-Graduação em Química. Belém, PA, Brasil. | en_US |
Affilliation | Universidade Federal do Pará. Programa de Pós-Graduação em Química. Belém, PA, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Maranhão. Pós Graduação em Zootecnia. São Luis, MA, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Universidade Federal do Pará. Programa de Pós-Graduação em Química. Belém, PA, Brasil. | en_US |
Affilliation | Universidade Federal do Pará. Programa de Pós-Graduação em Química. Belém, PA, Brasil. | en_US |
Affilliation | Universidade Federal de Santa Catarina. Departamento de Ciências Exatas e Educação. Blumenau, SC, Brasil. | en_US |
Subject | Bacillus subtilis | en_US |
Subject | Leishmania amazonensis | en_US |
Subject | Trypanosoma cruzi | en_US |
Subject | S-ribosylhomocysteine lyase | en_US |
Subject | Computational studies | en_US |
Subject | Phomoxanthone A | en_US |