Author | Johann, Susana | |
Author | Sá, Nívea P. | |
Author | Lima, Luciana Alves Rodrigues dos Santos | |
Author | Cisalpino, Patrícia Silva | |
Author | Cota, Betania Barros | |
Author | Alves, Tânia Maria de Almeida | |
Author | Siqueira Filho, Ezequias Pessoa de | |
Author | Zani, Carlos Leomar | |
Access date | 2012-10-04T17:29:47Z | |
Available date | 2012-10-04T17:29:47Z | |
Document date | 2010 | |
Citation | JOHANN, Susana et al. Antifungal activity of schinol and a new biphenyl compound isolated from Schinus terebinthifolius against the pathogenic fungus Paracoccidioides brasiliensis. Ann. Clin. Microbiol. Antimicrob, v. 9, n. 30, p. 1-6, 2010. | pt_BR |
ISSN | 14760711 | |
URI | https://www.arca.fiocruz.br/handle/icict/5641 | |
Language | eng | pt_BR |
Publisher | BioMed Central | pt_BR |
Rights | open access | pt_BR |
Title | Antifungal activity of schinol and a new biphenyl compound isolated from Schinus terebinthifolius against the pathogenic fungus Paracoccidioides brasiliensis | pt_BR |
Type | Article | pt_BR |
DOI | 10.1186/1476-0711-9-30 | |
Abstract | Background: The aim of this study was to isolate and identify the antifungal compounds from the extracts of Schinus terebinthifolius (Anacardiaceae) against clinical isolates of the pathogenic fungus Paracoccidioides brasiliensis.
Methods: The hexane and dichlomethane fractions from leaves and stems of S. terebinthifolius were fractionated using several chromatography techniques to afford four compounds.
Results: The compounds isolated from S. terebinthifolius were identified as schinol (1), a new biphenyl compound, namely, 4’-ethyl-4-methyl- 2,2’,6,6’-tetrahydroxy[1, 1’-biphenyl]-4,4’-dicarboxylate (2), quercetin (3), and kaempferol (4). Compounds 1 and 2 were active against different strains of P. brasiliensis, showing a minimal inhibitory concentration value against the isolate Pb B339 of 15.6 μg/ml. The isolate Pb 1578 was more sensitive to compound 1 with a MIC value of 7.5 μg/ml. Schinol presented synergistic effect only when combined with itraconazole. The compounds isolated from S. terebinthifolius were not able to inhibit cell wall synthesis or assembly using the sorbitol assay.
Conclusion: This work reveals for the first time the occurrence of compound 2 and discloses activity of compounds 1 and 2 against several clinical isolates of P. brasiliensis. These results justify further studies to clarify the mechanisms of action of these compounds. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, Brazil / Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, Brazil / Universidade Federal de São João Del-Rei. Campus Centro-Oeste Dona Lindu. Divinópolis, MG, Brazil. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Química de Produtos Naturais. Belo Horizonte, MG, Brazil. | pt_BR |
Subject | Paracoccidioidomycosis | pt_BR |
Subject | Antifungal activity | pt_BR |