Author | Freire, Mariana Lourenço | |
Author | Alves, Lindicy Leidicy | |
Author | Souza, Carolina Senra de | |
Author | Faria, Verônica | |
Author | Pedras, Mariana Junqueira | |
Author | Carvalho, Nara de Oliveira | |
Author | Andrade, Gláucia Queiroz | |
Author | Rabello, Ana Lucia Teles | |
Author | Avelar, Daniel Moreira | |
Author | Cota, Glaucia Fernandes | |
Access date | 2023-01-04T19:35:58Z | |
Available date | 2023-01-04T19:35:58Z | |
Document date | 2022 | |
Citation | FREIRE, Mariana Lourenço et al. Performance differences among commercially available antigen rapid tests for COVID-19 in Brazil. PLoS One, v. 17, n. 6, e0269997, 2022. doi: 10.1371/journal.pone.0269997. | en_US |
ISSN | 1932-6203 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/56326 | |
Language | eng | en_US |
Publisher | Public Library of Science | en_US |
Rights | open access | en_US |
Title | Performance differences among commercially available antigen rapid tests for COVID-19 in Brazil. | en_US |
Type | Article | en_US |
Abstract | A rapid and accurate diagnosis is a crucial strategy for containing the coronavirus disease (COVID-19) pandemic. Considering the obstacles to upscaling the use of RT–qPCR, rapid tests based on antigen detection (Ag-RDT) have become an alternative to enhance mass testing, reducing the time for a prompt diagnosis and virus spreading. However, the performances of several commercially available Ag-RDTs have not yet been evaluated in several countries. Here, we evaluate the performance of eight Ag-RDTs available in Brazil to diagnose COVID-19. Patients admitted to tertiary hospitals with moderate or mild COVID-19 symptoms and presenting risk factors for severe disease were included. The tests were performed using a masked protocol, strictly following the manufacturer’s recommendations and were compared with RT–qPCR. The overall sensitivity of the tests ranged from 9.8 to 81.1%, and specificity greater than 83% was observed for all the evaluated tests. Overall, slight or fair agreement was observed between Ag-RDTs and RT–PCR, except for the Ag-RDT COVID-19 (Acro Biotech), in which moderate agreement was observed. Lower sensitivity of Ag-RDTs was observed for patients with cycle threshold > 25, indicating that the sensitivity was directly affected by viral load, whereas the effect of the disease duration was unclear. Despite the lower sensitivity of Ag-RDTs compared with RT–qPCR, its easy fulfillment and promptness still justify its use, even at hospital admission. However, the main advantage of Ag-RDTs seems to be the possibility of increasing access to the diagnosis of COVID-19 in patients with a high viral load, allowing immediate clinical management and reduction of infectivity and community transmission. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Pesquisa Clínica e Políticas Públicas em Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Pesquisa Clínica e Políticas Públicas em Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Pesquisa Clínica e Políticas Públicas em Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brazil/Estado de Minas Gerais. Secretaria de Saúde. Coordenação Estadual de Laboratórios e Pesquisa em Vigilância da Subsecretaria de Vigilância em Saúde. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Pesquisa Clínica e Políticas Públicas em Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Pesquisa Clínica e Políticas Públicas em Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Núcleo de Ações e Pesquisa em apoio diagnóstico. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Núcleo de Ações e Pesquisa em apoio diagnóstico. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Pesquisa Clínica e Políticas Públicas em Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Pesquisa Clínica e Políticas Públicas em Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brazil | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto René Rachou. Pesquisa Clínica e Políticas Públicas em Doenças Infecciosas e Parasitárias. Belo Horizonte, MG, Brazil | en_US |