Author | Gama, Maiara do Valle Faria | |
Author | Moraes, Caroline Silva | |
Author | Gomes, Bruno | |
Author | Diaz-Albiter, Hector Manuel | |
Author | Mesquita, Rafael Dias | |
Author | Seabra Junior, Eloy | |
Author | Azambuja, Patrícia | |
Author | Garcia, Eloi de Souza | |
Author | Genta, Fernando Ariel | |
Access date | 2022-12-17T20:08:18Z | |
Available date | 2022-12-17T20:08:18Z | |
Document date | 2022 | |
Citation | GAMA, Maiara do Valle Faria et al. Structure and expression of Rhodnius prolixus GH18 chitinases and chitinase-like proteins: Characterization of the physiological role of RpCht7, a gene from subgroup VIII, in vector fitness and reproduction. Frontiers in Physiology, v. 13, 861620, p. 1 - 20, Oct. 2022. | en_US |
ISSN | 1664-042X | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/56028 | |
Language | eng | en_US |
Publisher | Frontiers Media | en_US |
Rights | open access | |
Subject in Portuguese | Quitinase | en_US |
Subject in Portuguese | Rhodnius prolixus | en_US |
Subject in Portuguese | RNAi | en_US |
Subject in Portuguese | Expressão genetica | en_US |
Subject in Portuguese | Oviposição | en_US |
Subject in Portuguese | Glicosídeo hidrolase família 18 | en_US |
Subject in Portuguese | Proteína semelhante à quitinase | en_US |
Title | Structure and expression of Rhodnius prolixus GH18 chitinases and chitinase-like proteins: Characterization of the physiological role of RpCht7, a gene from subgroup VIII, in vector fitness and reproduction | en_US |
Type | Article | |
DOI | 10.3389/fphys.2022.861620 | |
Abstract | Chitinases are enzymes responsible for the hydrolysis of glycosidic linkages
within chitin chains. In insects, chitinases are typically members of the
multigenic glycoside hydrolase family 18 (GH18). They participate in the
relocation of chitin during development and molt, and in digestion in
detritivores and predatory insects, and they control the peritrophic
membrane thickness. Chitin metabolism is a promising target for developing
vector control strategies, and knowledge of the roles of chitinases may reveal
new targets and illuminate unique aspects of their physiology and interaction
with microorganisms. Rhodnius prolixus is an important vector of Chagas
disease, which is caused by the parasite Trypanosoma cruzi. In this study,
we performed annotation and structural characterization of nine chitinase and
chitinase-like protein genes in the R. prolixus genome. The roles of their
corresponding transcripts were studied in more depth; their physiological
roles were studied through RNAi silencing. Phylogenetic analysis of coding
sequences showed that these genes belong to different subfamilies of
GH18 chitinases already described in other insects. The expression patterns
of these genes in different tissues and developmental stages were initially
characterized using RT-PCR. RNAi screening showed silencing of the gene
family members with very different efficiencies. Based on the knockdown
results and the general lack of information about subgroup VIII of GH18, the
RpCht7 gene was chosen for phenotype analysis. RpCht7 knockdown doubled the mortality in starving fifth-instar nymphs compared to dsGFP-injected
controls. However, it did not alter blood intake, diuresis, digestion, molting
rate, molting defects, sexual ratio, percentage of hatching, or average hatching
time. Nevertheless, female oviposition was reduced by 53% in RpCht7-silenced
insects, and differences in oviposition occurred within 14–20 days after a
saturating blood meal. These results suggest that RpCht7 may be involved in
the reproductive physiology and vector fitness of R. prolixus. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil / El Colegio de la Frontera Sur, ECOSUR, Campeche, Mexico. | en_US |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense, UFF, Niterói, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil. | en_US |
Subject | Chitinase | en_US |
Subject | Rhodnius prolixus | en_US |
Subject | RNAi | en_US |
Subject | Gene expression | en_US |
Subject | Oviposition | en_US |
Subject | Glycoside hydrolase family 18 | en_US |
Subject | Chitinase-like protein | en_US |