Author | Cavalcante, Liliane Tavares de Faria | |
Author | Fonseca, Guilherme Cordenonsi da | |
Author | Leon, Luciane Almeida Amado | |
Author | Salvio, Andreza Lemos | |
Author | Brustolini, Otávio José | |
Author | Gerber, Alexandra Lehmkuhl | |
Author | Guimarães, Ana Paula de Campos | |
Author | Marques, Carla Augusta Barreto | |
Author | Fernandes, Renan Amphilophio | |
Author | Ramos Filho, Carlos Henrique Ferreira | |
Author | Kader, Rafael Lopes | |
Author | Amaro, Marisa Pimentel | |
Author | Gonçalves, João Paulo da Costa | |
Author | Alves-Leon, Soniza Vieira | |
Author | Vasconcelos, Ana Tereza Ribeiro | |
Access date | 2022-12-16T10:16:23Z | |
Available date | 2022-12-16T10:16:23Z | |
Document date | 2022 | |
Citation | CAVALCANTE, Liliane Tavares de Faria et al. Buffy Coat Transcriptomic Analysis Reveals Alterations in Host Cell Protein Synthesis and Cell Cycle in Severe COVID-19 Patients. International Journal of Molecular Sciences, v. 23, 13588, p. 1 - 19, Nov. 2022. | en_US |
ISSN | 1422-0067 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/55988 | |
Language | eng | en_US |
Publisher | MDPI | en_US |
Rights | open access | |
Subject in Portuguese | COVID-19 | en_US |
Subject in Portuguese | SARS-CoV-2 | en_US |
Subject in Portuguese | RNA-seq | en_US |
Subject in Portuguese | lncRNA | en_US |
Subject in Portuguese | Biomarcadores | en_US |
Subject in Portuguese | Casaco felpudo | en_US |
Subject in Portuguese | FBL | en_US |
Subject in Portuguese | Pacientes de UTI | en_US |
Title | Buffy Coat Transcriptomic Analysis Reveals Alterations in Host Cell Protein Synthesis and Cell Cycle in Severe COVID-19 Patients | en_US |
Type | Article | |
DOI | 10.3390/ijms232113588 | |
Abstract | Transcriptome studies have reported the dysregulation of cell cycle-related genes and
the global inhibition of host mRNA translation in COVID-19 cases. However, the key genes and
cellular mechanisms that are most affected by the severe outcome of this disease remain unclear. For
this work, the RNA-seq approach was used to study the differential expression in buffy coat cells
of two groups of people infected with SARS-CoV-2: (a) Mild, with mild symptoms; and (b) SARS
(Severe Acute Respiratory Syndrome), who were admitted to the intensive care unit with the severe
COVID-19 outcome. Transcriptomic analysis revealed 1009 up-regulated and 501 down-regulated
genes in the SARS group, with 10% of both being composed of long non-coding RNA. Ribosome
and cell cycle pathways were enriched among down-regulated genes. The most connected proteins
among the differentially expressed genes involved transport dysregulation, proteasome degradation,
interferon response, cytokinesis failure, and host translation inhibition. Furthermore, interactome
analysis showed Fibrillarin to be one of the key genes affected by SARS-CoV-2. This protein interacts
directly with the N protein and long non-coding RNAs affecting transcription, translation, and
ribosomal processes. This work reveals a group of dysregulated processes, including translation and
cell cycle, as key pathways altered in severe COVID-19 outcomes. | en_US |
Affilliation | Laboratório Nacional de Computação Científica. Laboratório de Bioinformática. Petrópolis, RJ, Brasil. | en_US |
Affilliation | Laboratório Nacional de Computação Científica. Laboratório de Bioinformática. Petrópolis, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Universidade Federal do Estado do Rio de Janeiro. Laboratório de Neurociências Translacional. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Laboratório Nacional de Computação Científica. Laboratório de Bioinformática. Petrópolis, RJ, Brasil. | en_US |
Affilliation | Laboratório Nacional de Computação Científica. Laboratório de Bioinformática. Petrópolis, RJ, Brasil. | en_US |
Affilliation | Laboratório Nacional de Computação Científica. Laboratório de Bioinformática. Petrópolis, RJ, Brasil. | en_US |
Affilliation | Laboratório Nacional de Computação Científica. Laboratório de Bioinformática. Petrópolis, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Universidade Federal do Estado do Rio de Janeiro. Laboratório de Neurociências Translacional. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Universidade Estadual do Rio de Janeiro. Instituto Biomédico. Laboratório de Neurociências Translacionais. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Universidade Federal do Estado do Rio de Janeiro. Laboratório de Neurociências Translacional. Rio de Janeiro, RJ, Brasil / Yale New Haven Hospital, New Haven, CT 06510, USA. | en_US |
Affilliation | Universidade Federal do Estado do Rio de Janeiro. Laboratório de Neurociências Translacional. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Laboratório Nacional de Computação Científica. Laboratório de Bioinformática. Petrópolis, RJ, Brasil. | en_US |
Subject | COVID-19 | en_US |
Subject | SARS-CoV-2 | en_US |
Subject | RNA-seq | en_US |
Subject | lncRNA | en_US |
Subject | Biomarkers | en_US |
Subject | Buffy coat | en_US |
Subject | FBL | en_US |
Subject | ICU patients | en_US |