Author | Belitardo, Emilia M. M. Andrade | |
Author | Nery Jr, Nivison | |
Author | Ticona, Juan P. Aguilar | |
Author | Portilho, Moyra Machado | |
Author | Mello, Iago O | |
Author | Ribeiro, Guilherme S. | |
Author | Reis, Mitermayer G. | |
Author | Costa, Federico | |
Author | Cummings, Derek A. T. | |
Author | Ko, Albert I. | |
Author | Fofana, Mariam O. | |
Access date | 2022-12-15T18:36:16Z | |
Available date | 2022-12-15T18:36:16Z | |
Document date | 2022 | |
Citation | BELITARDO, Emilia M. M. Andrade et al. Reliable estimation of SARS-CoV-2 anti-spike protein IgG titers from single dilution optical density values in serologic surveys. Diagnostic Microbiology and Infectious Disease, v. 104, n. 4, p. 1-12, 2022. | en_US |
ISSN | 0732-8893 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/55983 | |
Sponsorship | National Institutes of Health.
UK Medical Research Council.
Wellcome Trust.
Coordenação Brasileira de Aperfeiçoamento de Pessoal de Nível Superior (CAPES).
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).
Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB).
Bill & Melinda Gates Foundation.
American Society of Tropical Medicine and Hygiene .
Sendas Family Fund at the Yale School of Public Health. | en_US |
Language | eng | en_US |
Publisher | Elsevier | en_US |
Rights | open access | en_US |
Subject in Portuguese | SARS-CoV-2 | en_US |
Subject in Portuguese | Sorologia | en_US |
Subject in Portuguese | Anticorpos | en_US |
Subject in Portuguese | Densidade ótica | en_US |
Subject in Portuguese | Títulos | en_US |
Title | Reliable estimation of SARS-CoV-2 anti-spike protein IgG titers from single dilution optical density values in serologic surveys | en_US |
Type | Article | en_US |
DOI | 10.1016/j.diagmicrobio.2022.115807 | |
Abstract | Reliable and scalable seroepidemiology methods are needed to estimate SARS-CoV-2 incidence and monitor the dynamics of population-level immunity as the pandemic evolves. We aimed to evaluate the reliability of SARS-CoV-2 normalized ELISA optical density (nOD) at a single dilution compared to titers derived from serial dilutions. We conducted serial serosurveys within a community-based cohort in Salvador, Brazil. AntiS IgG ELISA (Euroimmun AG) was performed with 5 serial 3-fold dilutions of paired sera from 54 participants. Changes in nOD reliably predicted increases and decreases in titers (98.1% agreement, k = 95.8%). Fitting the relationship between nOD and interpolated titers to a log-log curve yields highly accurate predictions of titers (r2 = 0.995) and changes in titers (r2 = 0.975), using only 1 to 2 dilutions. This approach can significantly reduce the time, labor and resources needed for large-scale erosurveys to ascertain population-level changes in exposure and immunity . | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, United States. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Saúde Coletiva. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, United States. | en_US |
Affilliation | University of Florida. Department of Biology. Gainesville, FL, United States / University of Florida. Emerging Pathogens Institute. Gainesville, FL, United States. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, United States. | en_US |
Affilliation | Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, United States | en_US |
Subject | SARS-CoV-2 | en_US |
Subject | Serology | en_US |
Subject | Antibody | en_US |
Subject | Optical density | en_US |
Subject | Titers | en_US |
DeCS | SARS-CoV-2 | en_US |
DeCS | Sorologia | en_US |
DeCS | Anticorpos | en_US |