Author | Furtado, Nathália Dias | |
Author | Gómez, Mariela Martínez | |
Author | Mello, Iasmim Silva de | |
Author | Fernandes, Déberli Ruiz | |
Author | Bonaldo, Myrna Cristina | |
Access date | 2022-12-15T16:57:15Z | |
Available date | 2022-12-15T16:57:15Z | |
Document date | 2022 | |
Citation | FURTADO, Nathália Dias et al. Phenotypic and Genetic Studies of the Viral Lineage Associated with the Recent Yellow Fever Outbreak in Brazil. Viruses, v. 14, 1818, p. 1 - 14, Aug. 2022. | en_US |
ISSN | 1999-4915 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/55977 | |
Language | eng | en_US |
Publisher | MDPI | en_US |
Rights | open access | |
Subject in Portuguese | Surto do vírus da febre amarela | en_US |
Subject in Portuguese | Sudeste do Brasil | en_US |
Subject in Portuguese | Polimorfismos de aminoácidos | en_US |
Subject in Portuguese | NS3 | en_US |
Subject in Portuguese | NS5 | en_US |
Subject in Portuguese | Virulência | en_US |
Title | Phenotypic and Genetic Studies of the Viral Lineage Associated with the Recent Yellow Fever Outbreak in Brazil | en_US |
Type | Article | |
DOI | 10.3390/v14081818 | |
Abstract | Yellow fever virus (YFV) caused an outbreak in the Brazilian Southeast from 2016 to 2019,
of the most significant magnitude since the 1900s. An investigation of the circulating virus revealed
that most of the genomes detected in this period carried nine unique amino acid polymorphisms,
with eight located in the non-structural proteins NS3 and NS5, which are pivotal for viral replication.
To elucidate the effect of these amino acid changes on viral infection, we constructed viruses carrying
amino acid alterations in NS3 and NS5, performed infection in different cells, and assessed their
neurovirulence in BALB/c mice and infected AG129 mice. We observed that the residues that
compose the YFV 2016–2019 molecular signature in the NS5 protein might have been related to
an attenuated phenotype, and that the alterations in the NS3 protein only slightly affected viral
infection in AG129 mice, increasing to a low extent the mortality rate of these animals. These results
contributed to unveiling the role of specific naturally occurring amino acid changes in the circulating
strain of YFV in Brazil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Flavivírus. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Flavivírus. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Flavivírus. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Flavivírus. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Flavivírus. Rio de Janeiro, RJ, Brasil. | en_US |
Subject | Yellow fever virus outbreak | en_US |
Subject | Southeastern Brazil | en_US |
Subject | Amino acid polymorphisms | en_US |
Subject | NS3 | en_US |
Subject | NS5 | en_US |
Subject | Virulence | en_US |