Author | Santos, Debora Ribeiro de Souza | |
Author | Bianco, Kayo | |
Author | Clementino, Maysa Beatriz Mandetta | |
Author | Rivera Dávila, Alberto Martín | |
Author | Filippis, Ivano de | |
Access date | 2022-11-30T19:01:24Z | |
Available date | 2022-11-30T19:01:24Z | |
Document date | 2022 | |
Citation | SANTOS, Debora Ribeiro de Souza et al. Characterisation of Neisseria meningitidis cc11/ET-15 variant by whole genome sequencing. Memórias do Instituto Oswaldo Cruz, v. 117, e220118. p. 1 - 10, Sep. 2022. | en_US |
ISSN | 0074-0206 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/55844 | |
Language | eng | en_US |
Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. | en_US |
Rights | open access | |
Subject in Portuguese | Sequenciamento de genoma completo (WGS) | en_US |
Subject in Portuguese | Neisseria meningitidis cc11/ET-15 | en_US |
Title | Characterisation of Neisseria meningitidis cc11/ET-15 variant by whole genome sequencing | en_US |
Type | Article | |
DOI | 10.1590/0074-02760220118 | |
Abstract | BACKGROUND Neisseria meningitidis strains belonging to clonal complex 11 is the cause of numerous outbreaks and epidemics
in the United States, Canada and Europe, accounting for 49.5% of cases of meningococcal disease caused by serogroup C
worldwide. In Brazil, it is the second most frequent clonal complex within this serogroup. The genetic characterisation of cc11/
ET-15 variants is important for the epidemiological monitoring of meningococcal disease, through the identification of circulating
epidemic clones, to support specific actions of Health Surveillance aiming outbreaks control.
OBJECTIVES: The objective of this study was to identify features in the genome of cc11/ET-15 clones through whole-genome sequencing (WGS), that differ from cc11/non-ET-15 strains that could explain their virulence. METHODS: The whole genome of three cc11/ET-15 representative strains were sequenced with a minimum coverage of 100X with the MiSeq System and compared to the genome of cc11/non-ET-15 strains. RESULTS: Genome analysis of cc11/ET-15 variants showed the presence of resistance factors, mobile genetic elements and virulence factors not found in cc11/non-ET-15 strains. MAIN CONCLUSIONS: Our results show that these strains carry virulence factors not identified in cc11/non-ET-15 strains, which could explain the high lethality rates attributed to this clone worldwide. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil. | |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil. | |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil. | |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Rio de Janeiro, RJ, Brasil. | |
Subject | Whole-genome sequencing (WGS) | en_US |
Subject | Neisseria meningitidis cc11/ET-15 | en_US |