Author | Clasen, Milan A. | |
Author | Kurt, Louise U. | |
Author | Santos, Marlon D. M. | |
Author | Lima, Diogo B. | |
Author | Liu, Fan | |
Author | Gozzo, Fabio C. | |
Author | Barbosa, Valmir C. | |
Author | Carvalho, Paulo Costa | |
Access date | 2022-11-28T14:00:33Z | |
Available date | 2022-11-28T14:00:33Z | |
Document date | 2022 | |
Citation | CLASEN, Milan A. et al. Increasing confidence in proteomic spectral deconvolution through mass defect. Bioinformatics, p. 1–2, 2022. | en_US |
ISSN | 1460-2059 | en_US |
URI | https://www.arca.fiocruz.br/handle/icict/55813 | |
Language | por | en_US |
Publisher | Oxford | en_US |
Rights | open access | en_US |
Subject in Portuguese | Y.A.D.A. 3.0 | en_US |
Title | Increasing confidence in proteomic spectral deconvolution through mass defect | en_US |
Type | Article | en_US |
DOI | 10.1093/bioinformatics/btac638 | |
Abstract | Motivation: Confident deconvolution of proteomic spectra is critical for several applications such as sequencing, cross-linking mass spectrometry and handling chimeric mass spectra. Results: In general, all deconvolution algorithms may eventually report mass peaks that are not compatible with the chemical formula of any peptide. We show how to remove these artifacts by considering their mass defects. We introduce Y.A.D.A. 3.0, a fast deconvolution algorithm that can remove peaks with unacceptable mass defects. Our approach is effective for polypeptides with less than 10 kDa, and its essence can be easily incorporated into any deconvolution algorithm. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica Estrutural e Computacional. Curitiba, PR, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica Estrutural e Computacional. Curitiba, PR, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica Estrutural e Computacional. Curitiba, PR, Brasil. | en_US |
Affilliation | Department of Structural Biology. Leibniz - Forschungsinstitut für Molekulare Pharmakologie, Berlin, Germany. | en_US |
Affilliation | Department of Structural Biology. Leibniz - Forschungsinstitut für Molekulare Pharmakologie, Berlin, Germany. | en_US |
Affilliation | Universidade Estadual de Campinas. Dalton Mass Spectrometry Laboratory. Campinas, Brasil. | en_US |
Affilliation | Universidade Federal do Rio de Janeiro. Programa em Engenharia de Sistemas e Computação. Rio de Janeiro, RJ, Brasil. | en_US |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica Estrutural e Computacional. Curitiba, PR, Brasil. | en_US |
Subject | Proteomics | en_US |
Subject | Mass Spectrometry | en_US |
Subject | Algorithms | en_US |
Subject in Spanish | Proteómica | en_US |
Subject in Spanish | Espectrometría de Masas | en_US |
Subject in Spanish | Algoritmos | en_US |
Subject in French | Protéomique | en_US |
Subject in French | Spectrométrie de masse | en_US |
Subject in French | Algorithmes | en_US |
DeCS | Proteômica | en_US |
DeCS | Espectrometria de Massas | en_US |
DeCS | Algoritmos | en_US |