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2025-02
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- IOC - Artigos de Periódicos [12490]
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EVALUATION OF VITEK® 2 AND MALDI-TOF/MS AUTOMATED METHODOLOGIES IN THE IDENTIFICATION OF ATYPICAL LISTERIA SPP. ISOLATED FROM FOOD IN DIFFERENT REGIONS OF BRAZIL
Listeria monocytogenes
Metodologias automatizadas
PCR
Espectrometria de massa
Author
Affilliation
Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro. Departamento de Alimentação. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.
Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro. Departamento de Alimentação. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Faculdade de Medicina Veterinária. Niterói, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.
Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro. Departamento de Alimentação. Rio de Janeiro, RJ, Brasil.
Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro. Departamento de Alimentação. Rio de Janeiro, RJ, Brasil / Universidade Federal Fluminense. Faculdade de Medicina Veterinária. Niterói, RJ. Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Zoonoses Bacterianas. Rio de Janeiro, RJ, Brasil.
Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro. Departamento de Alimentação. Rio de Janeiro, RJ, Brasil.
Abstract
Listeria monocytogenes is a pathogen responsible for listeriosis, a foodborne disease with high mortality rates
(20–30%). It mainly affects the elderly, pregnant women, and immunocompromised people. Although not
pathogenic, the isolation and identification of Listeria innocua are critical since they can indicate L. monocytogenes’
presence as they are closely related and widely distributed in the environment and food processing
plants. The objective of this study was to evaluate the effectiveness of the automated methods VITEK® 2 and
MALDI-TOF/MS in identifying 94 strains of the genus Listeria with atypical identification profile. The resulting
identification by Polymerase Chain Reaction (PCR), using specific primers for the most common species of Listeria,
was considered the correct identification and presented a total of 31 strains identified as Listeria innocua
(LI), 54 as L. monocytogenes (LM), 8 as Listeria welshimeri (LW) and 1 as Listeria grayi (LG). The VITEK® 2
automated system correctly identified, on average, 79% of the LI strains, 16% of the LM strains, and 88.0% of the
LW strains. In the analysis by MALDI-TOF/MS, on average, 73% of LM strains were correctly identified, few LW
strains were correctly identified, and all LI strains were incorrectly identified. Both VITEK® 2 and MALDI-TOF/
MS correctly identified the LG strain in both analyzes. The results demonstrate that automated methodologies
could not discriminate atypical strains of the Listeria genus and point to the need for the use of complementary
tests, such as PCR and chromogenic media, for the correct identification of these strains.
Keywords in Portuguese
Listeria innocuaListeria monocytogenes
Metodologias automatizadas
PCR
Espectrometria de massa
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