Author | Krmeská, Veronika | |
Author | Aggio, Juliana Bernardi | |
Author | Nylén, Susanne | |
Author | Wowk, Pryscilla Fanini | |
Author | Rothfuchs, Antonio Gigliotti | |
Access date | 2022-06-20T17:30:11Z | |
Available date | 2022-06-20T17:30:11Z | |
Document date | 2022 | |
Citation | KRMESKÁ, Veronika et al. Cyclooxygenase-derived prostaglandin E2 drives IL-1-Independent Mycobacterium bovis Bacille Calmette-Guérin-Triggered Skin Dendritic Cell Migration to Draining Lymph Node. the Journal of Immunology, p. 2549–2557, 2022. | pt_BR |
ISSN | 1550-6606 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/53376 | |
Language | por | pt_BR |
Publisher | American Association of Immunologists | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | BCG | pt_BR |
Subject in Portuguese | PGE2 | pt_BR |
Subject in Portuguese | IL-1 | pt_BR |
Title | Cyclooxygenase-Derived Prostaglandin E2 Drives IL-1-Independent Mycobacterium bovis Bacille Calmette-Guérin-Triggered skin dendritic cell migration to draining lymph node | pt_BR |
Type | Article | pt_BR |
DOI | 10.4049/jimmunol.2100981 | |
Abstract | Inoculation of Mycobacterium bovis Bacille Calmette-Guerin (BCG) in the skin mobilizes local dendritic cells (DC) to the draining lymph node (dLN) in a process that remains incompletely understood. In this study, a mouse model of BCG skin infection was used to investigate mechanisms of skin DC migration to dLNs. We found enhanced transcription of cyclooxygenase (COX)-2 and production of COX-derived PGE2 early after BCG infection in skin. Animals treated with antagonists for COX or the PGE2 receptors EP2 and EP4 displayed a marked reduction in the entry of skin DCs and BCG to dLNs, uncovering an important contribution of COX-derived PGE2 in this migration process. In addition, live BCG bacilli were needed to invoke DC migration through this COX-PGE2 pathway. Having previously shown that IL-1R partially regulates BCG-induced relocation of skin DCs to dLNs, we investigated whether PGE2 release was under control of IL-1. Interestingly, IL-1R ligands IL-1a/b were not required for early transcription of COX-2 or production of PGE2 in BCG-infected skin, suggesting that the DC migration-promoting role of PGE2 is independent of IL-1a/b in our model. In DC adoptive transfer experiments, EP2/EP4, but not IL-1R, was needed on the moving DCs for full-fledged migration, supporting different modes of action for PGE2 and IL-1a/b. In summary, our data highlight an important role for PGE2 in guiding DCs to dLNs in an IL-1 independent manner. | pt_BR |
Affilliation | Department of Microbiology. Tumor and Cell Biology. Karolinska Institutet. Stockholm, Sweden. | pt_BR |
Affilliation | Department of Microbiology. Tumor and Cell Biology. Karolinska Institutet. Stockholm, Sweden. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Department of Microbiology. Tumor and Cell Biology. Karolinska Institutet. Stockholm, Sweden. | pt_BR |
Affilliation | Department of Microbiology. Tumor and Cell Biology. Karolinska Institutet. Stockholm, Sweden. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Department of Microbiology. Tumor and Cell Biology. Karolinska Institutet. Stockholm, Sweden. | pt_BR |
Subject | Mycobacterium bovis | pt_BR |
Subject | Dendritic Cells | pt_BR |
Subject | Lymph Nodes | pt_BR |
Subject | Cyclooxygenase-2 | pt_BR |
Subject | Dinoprostone | pt_BR |
Subject | Interleukin-1 | pt_BR |
Subject in Spanish | Mycobacterium bovis | pt_BR |
Subject in Spanish | Células Dendríticas | pt_BR |
Subject in Spanish | Ganglios Linfáticos | pt_BR |
Subject in Spanish | Ciclooxigenasa 2 | pt_BR |
Subject in Spanish | Interleucina-1 | pt_BR |
DeCS | Mycobacterium bovis | pt_BR |
DeCS | Bacilo de Calmette-Guérin | pt_BR |
DeCS | Células Dendríticas | pt_BR |
DeCS | Linfonodos | pt_BR |
DeCS | Cicloxigenase 2 | pt_BR |
DeCS | Dinoprostona | pt_BR |
DeCS | Interleucina-1 | pt_BR |