Author | Rosón, Juliana Nunes | |
Author | Vitarelli, Marcela de Oliveira | |
Author | Costa-Silva, Héllida Marina | |
Author | Pereira, Kamille Schmitt | |
Author | Pires, David da Silva | |
Author | Lopes, Leticia de Sousa | |
Author | Cordeiro, Barbara | |
Author | Kraus, Amelie J. | |
Author | Cruz, Karin Navarro Tozzi | |
Author | Calderano, Simone Guedes | |
Author | Fragoso, Stenio Perdigão | |
Author | Siegel, T. Nicolai | |
Author | Elias, Maria Carolina | |
Author | Cunha, Julia Pinheiro Chagas da | |
Access date | 2022-05-26T18:29:50Z | |
Available date | 2022-05-26T18:29:50Z | |
Document date | 2022 | |
Citation | ROSÓN, Juliana Nunes et al. H2B.V demarcates divergent strand-switch regions, some tDNA loci, and genome compartments in Trypanosoma cruzi and affects parasite differentiation and host cell invasion. PLoS Pathog, v. 18, n. 2, p. 1–27, 2022. | pt_BR |
ISSN | 1553-7374 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/52938 | |
Language | por | pt_BR |
Publisher | Public Library of Science | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Trypanosoma cruzi | pt_BR |
Title | H2B.V demarcates divergent strand-switch regions, some tDNA loci, and genome compartments in Trypanosoma cruzi and affects parasite differentiation and host cell invasion | pt_BR |
Type | Article | pt_BR |
DOI | 10.1371/ journal.ppat.1009694 | |
Abstract | Histone variants play a crucial role in chromatin structure organization and gene expression. Trypanosomatids have an unusual H2B variant (H2B.V) that is known to dimerize with the variant H2A.Z generating unstable nucleosomes. Previously, we found that H2B.V protein is enriched in tissue-derived trypomastigote (TCT) life forms, a nonreplicative stage of Trypanosoma cruzi, suggesting that this variant may contribute to the differences in chromatin structure and global transcription rates observed among parasite life forms. Here, we performed the first genome-wide profiling of histone localization in T. cruzi using epimastigotes and TCT life forms, and we found that H2B.V was preferentially located at the edges of divergent transcriptional strand switch regions, which encompass putative transcriptional start regions; at some tDNA loci; and between the conserved and disrupted genome compartments, mainly at trans-sialidase, mucin and MASP genes. Remarkably, the chromatin of TCT forms was depleted of H2B.V-enriched peaks in comparison to epimastigote forms. Interactome assays indicated that H2B.V associated specifically with H2A.Z, bromodomain factor 2, nucleolar proteins and a histone chaperone, among others. Parasites expressing reduced H2B.V levels were associated with higher rates of parasite differentiation and mammalian cell infectivity. Taken together, H2B.V demarcates critical genomic regions and associates with regulatory chromatin proteins, suggesting a scenario wherein local chromatin structures associated with parasite differentiation and invasion are regulated during the parasite life cycle. | pt_BR |
Affilliation | Instituto Butantan. Laboratório de Ciclo Celular. São Paulo, SP, Brasil. / Instituto Butantan. Centro de Toxinas, Resposta Imune e Sinalização Celular - CeTICS. São Paulo, SP, Brasil. / Universidade Federal de São Paulo. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Instituto Butantan. Laboratório de Ciclo Celular. São Paulo, SP, Brasil. / Instituto Butantan. Centro de Toxinas, Resposta Imune e Sinalização Celular - CeTICS. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Instituto Butantan. Laboratório de Ciclo Celular. São Paulo, SP, Brasil. / Instituto Butantan. Centro de Toxinas, Resposta Imune e Sinalização Celular - CeTICS. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Universidade Federal do Paraná. Departamento de Bioprocessos e Biotecnologia. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Molecular e Sistêmica de Tripanossomatídeos. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Instituto Butantan. Laboratório de Ciclo Celular. São Paulo, SP, Brasil. / Instituto Butantan. Centro de Toxinas, Resposta Imune e Sinalização Celular - CeTICS. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Instituto Butantan. Laboratório de Ciclo Celular. São Paulo, SP, Brasil. / Instituto Butantan. Centro de Toxinas, Resposta Imune e Sinalização Celular - CeTICS. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Instituto Butantan. Laboratório de Ciclo Celular. São Paulo, SP, Brasil. / Instituto Butantan. Centro de Toxinas, Resposta Imune e Sinalização Celular - CeTICS. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Division of Experimental Parasitology. Faculty of Veterinary Medicine. Ludwig-Maximilians-Universität in Munich. Munich, Germany. / Biomedical Center. Division of Physiological Chemistry. Faculty of Medicine. Ludwig-Maximilians-Universität in Munch. Munich, Germany. | pt_BR |
Affilliation | Instituto Butantan. Laboratório de Ciclo Celular. São Paulo, SP, Brasil. / Instituto Butantan. Centro de Toxinas, Resposta Imune e Sinalização Celular - CeTICS. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Instituto Butantan. Laboratório de Ciclo Celular. São Paulo, SP, Brasil. / Instituto Butantan. Centro de Toxinas, Resposta Imune e Sinalização Celular - CeTICS. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Universidade Federal do Paraná. Departamento de Bioprocessos e Biotecnologia. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Molecular e Sistêmica de Tripanossomatídeos. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Division of Experimental Parasitology. Faculty of Veterinary Medicine. Ludwig-Maximilians-Universität in Munich. Munich, Germany. / Biomedical Center. Division of Physiological Chemistry. Faculty of Medicine. Ludwig-Maximilians-Universität in Munch. Munich, Germany. | pt_BR |
Affilliation | Instituto Butantan. Laboratório de Ciclo Celular. São Paulo, SP, Brasil. / Instituto Butantan. Centro de Toxinas, Resposta Imune e Sinalização Celular - CeTICS. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Instituto Butantan. Laboratório de Ciclo Celular. São Paulo, SP, Brasil. / Instituto Butantan. Centro de Toxinas, Resposta Imune e Sinalização Celular - CeTICS. São Paulo, SP, Brasil. | pt_BR |
Subject | Histones | pt_BR |
Subject | High Mobility Group Proteins | pt_BR |
Subject | Cell Differentiation | pt_BR |
Subject | Host-Parasite Interactions | pt_BR |
Subject in Spanish | Histonas | pt_BR |
Subject in Spanish | Proteínas del Grupo de Alta Movilidad | pt_BR |
Subject in Spanish | Diferenciación Celular | pt_BR |
Subject in Spanish | Interacciones Huésped-Parásitos | pt_BR |
Subject in French | Histone | pt_BR |
Subject in French | Protéines HMG | pt_BR |
Subject in French | Différenciation cellulaire | pt_BR |
Subject in French | Interactions hôte-parasite | pt_BR |
DeCS | Histonas | pt_BR |
DeCS | Proteínas de Grupo de Alta Mobilidade | pt_BR |
DeCS | Interações Hospedeiro-Parasita | pt_BR |
DeCS | Diferenciação Celular | pt_BR |