Author | Carvalho, Augusto M. | |
Author | Viana, Sayonara M. | |
Author | Andrade, Bruno B. | |
Author | Oliveira, Fabiano | |
Author | Valenzuela, Jesus G. | |
Author | Carvalho, Edgar M. | |
Author | Oliveira, Camila I. de | |
Access date | 2022-05-06T14:42:02Z | |
Available date | 2022-05-06T14:42:02Z | |
Document date | 2022 | |
Citation | CARVALHO, Augusto M. et al. Immune response to LinB13, a lutzomyia intermedia salivary protein correlates with disease severity in tegumentary leishmaniasis. Clinical Infectious Diseases, p. 1-29, 2022. | pt_BR |
ISSN | 1537-6591 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/52557 | |
Sponsorship | PROEP-IGM-Fiocruz Bahia. Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). Programa de Pesquisa Intramural do Instituto Nacional de Alergia e Doenças Infecciosas | pt_BR |
Language | eng | pt_BR |
Publisher | Oxford University Press | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Gravidade da doença | pt_BR |
Subject in Portuguese | Leishmania braziliensis | pt_BR |
Subject in Portuguese | Leishmaniose cutânea | pt_BR |
Subject in Portuguese | Leishmaniose disseminada | pt_BR |
Subject in Portuguese | Saliva do flebotomíneo | pt_BR |
Title | Immune response to LinB13, a lutzomyia intermedia salivary protein correlates with disease severity in tegumentary leishmaniasis | pt_BR |
Type | Article | pt_BR |
DOI | 10.1093/cid/ciac258 | |
Abstract | Background: We have previously shown that seropositivity to rLinB-13, a salivary protein from Lutzomyia intermedia, predicted sand fly exposure and was associated with increased risk of developing cutaneous leishmaniasis (CL). Methods: Herein, we investigated the cellular immune response to saliva from Lu. intermedia, using rLinB-13 as a surrogate antigen in naturally exposed individuals presenting positive serology to LinB-13. We also investigated the response to rLinB-13 in leishmaniasis patients, displaying active ulcers and positive PCR for L. braziliensis. Results: Peripheral blood mononuclear cells (PBMCs) stimulated in vitro with rLinB-13 secreted elevated levels of IL-10, IL-4, IL-1β, IL-1α, IL-6 and chemokines (CCL3, CCL4, CCL5 and CXCL5). CL, and disseminated leishmaniasis (DL) patients displayed a significantly higher IgG response to rLinB-13, compared to healthy subjects and anti-rLinB-13 IgG was positively correlated with the number of lesions in DL patients. Positive serology to rLinB-13 was also associated with chemotherapy failure. PBMCs from DL patients stimulated with rLINB-13 secreted significantly higher levels IL-10 and IL-1β compared to CL individuals. Conclusions: In this study, we observed an association between humoral and cellular immune response to the sand fly salivary protein rLinB-13 and disease severity in tegumentary leishmaniasis. This study brings evidence that immunity to rLinB-13 influences disease outcome in L. braziliensis infection and results indicate that positive serology to rLinB-13 IgG can be employed as marker of DL, an emerging and severe form of disease caused by L. braziliensis. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. National Institute of Allergy and Infectious Diseases. National Institutes of Health. Rockville, Maryland, USA. | pt_BR |
Affilliation | Vector Molecular Biology Section. Laboratory of Malaria and Vector Research. National Institute of Allergy and Infectious Diseases. National Institutes of Health. Rockville, Maryland, USA. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil / Universidade Federal da Bahia. Serviço de Imunologia do Hospital Universitário Professor Edgard Santos. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Salvador, BA, Brasil. | pt_BR |
Subject | Disease severity | pt_BR |
Subject | Leishmania braziliensis | pt_BR |
Subject | Cutaneous leishmaniasis | pt_BR |
Subject | Disseminated leishmaniasis | pt_BR |
Subject | Sand fly saliva | pt_BR |
DeCS | Leishmania braziliensis | pt_BR |
DeCS | Leishmaniose cutânea | pt_BR |
DeCS | Índice de Gravidade de Doença | pt_BR |