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https://www.arca.fiocruz.br/handle/icict/51174
IN VITRO AND IN VIVO MODELS FOR STUDYING SARS-COV-2, THE ETIOLOGICAL AGENT RESPONSIBLE FOR COVID-19 PANDEMIC
Author
Affilliation
Oswaldo Cruz Foundation. Aggeu Magalhães Institute. Department of Virology. Recife, PE, Brazil / Federal University of Uberlandia. Rodents Animal Facilities Complex. Uberlandia, MG, Brazil.
Federal Rural University of Pernambuco. Department of Chemistry. Recife, PE, Brazil.
Oswaldo Cruz Foundation. Aggeu Magalhães Institute. Department of Virology. Recife, PE, Brazil.
Federal University of Uberlandia. Rodents Animal Facilities Complex. Uberlandia, MG, Brazil.
Federal Rural University of Pernambuco. Department of Chemistry. Recife, PE, Brazil.
Oswaldo Cruz Foundation. Aggeu Magalhães Institute. Department of Virology. Recife, PE, Brazil.
Federal Rural University of Pernambuco. Department of Chemistry. Recife, PE, Brazil.
Oswaldo Cruz Foundation. Aggeu Magalhães Institute. Department of Virology. Recife, PE, Brazil.
Federal University of Uberlandia. Rodents Animal Facilities Complex. Uberlandia, MG, Brazil.
Federal Rural University of Pernambuco. Department of Chemistry. Recife, PE, Brazil.
Oswaldo Cruz Foundation. Aggeu Magalhães Institute. Department of Virology. Recife, PE, Brazil.
Abstract
The emergence and rapid worldwide spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has prompted the scientific community to rapidly develop in vitro and in vivo models that could be applied in COVID-19 research. In vitro models include two-dimensional (2D) cultures of immortalized cell lines or primary cells and three-dimensional (3D) cultures derived from lung, alveoli, bronchi, and other organs. Although cell-based systems are economic and allow strict control of experimental variables, they do not always resemble physiological conditions. Thus, several in vivo models are being developed, including different strains of mice, hamsters, ferrets, dogs, cats, and non-human primates. In this review, we summarize the main models of SARS-CoV-2 infection developed so far and discuss their advantages, drawbacks and main uses.
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