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ALTENUSIN, A BIPHENYL ISOLATED FROM THE ENDOPHYTIC FUNGUS ALTERNARIA SP., INHIBITS TRYPANOTHIONE REDUCTASE FROM TRYPANOSOMA CRUZI
endophytic fungus
Alternaria sp.
trypanothione reductase
Trypanosoma cruzi
Leishmania amazonensis
Author
Affilliation
Fundacao Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Quımica de Produtos Naturais. Belo Horizonte, MG, Brazil
Universidade Federal de Ouro Preto. Instituto de Ciencias Exatas e Biologicas. Laboratorio de Microbiologia. MG, Brazil
Fundacao Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Pesquisas Clınicas. Belo Horizonte, MG, Brazil
Fundacao Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Pesquisas Clınicas. Belo Horizonte, MG, Brazil
Fundacao Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Quımica de Produtos Naturais. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil
Fundacao Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Quımica de Produtos Naturais. Belo Horizonte, MG, Brazil
Universidade Federal de Ouro Preto. Instituto de Ciencias Exatas e Biologicas. Laboratorio de Microbiologia. MG, Brazil
Fundacao Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Pesquisas Clınicas. Belo Horizonte, MG, Brazil
Fundacao Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Pesquisas Clınicas. Belo Horizonte, MG, Brazil
Fundacao Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Quımica de Produtos Naturais. Belo Horizonte, MG, Brazil
Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Microbiologia. Belo Horizonte, MG, Brazil
Fundacao Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Laboratorio de Quımica de Produtos Naturais. Belo Horizonte, MG, Brazil
Abstract
Parasitic protozoan species belonging to the genera Trypanosoma and Leishmania are the etiological agents of several diseases in tropical areas of the world, for which there is an urgent need for effective and affordable treatment. In this regard, we are screening the Brazilian biodiversity, especially its flora and mycota, for natural products that could serve as leads for drug development against these diseases. Trypanothione reductase (TR) is an enzyme involved in the protection of Trypanosoma and Leishmania species against oxidative stress, and is considered to be a validated drug target. The endophytic fungus Alternaria sp. (UFMGCB55) was isolated from the plant Trixis vauthieri DC (Asteraceae), known to contain trypanocidal compounds. The organic extract of the culture of Alternaria sp. was able to inhibit TR by 99%, when tested at 20 mu g mL(-1). Fractionation of the extract identified altenusin, a biphenyl derivative with an IC50 value of 4.3 +/- 0.3 mu M in the TR assay. This compound is the first in its class to have shown TR inhibitory activity, opening new perspectives for the design of more effective derivatives that could serve as drug leads for new chemotherapeutic agents to treat trypanosomiasis and leishmaniasis
Keywords
altenusinendophytic fungus
Alternaria sp.
trypanothione reductase
Trypanosoma cruzi
Leishmania amazonensis
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