Author | Lima, Israel Diniz | |
Author | Rosa, Pablo Rodrigo da | |
Author | Silva Junior, Elias Barbosa da | |
Author | Oliveira, Joyce Cristina Guimarães de | |
Author | Freitas, Elisangela Oliveira de | |
Author | Nascimento, Danielle de Oliveira | |
Author | Morrot, Alexandre | |
Author | Nimrichter, Leonardo | |
Author | Previato, José Osvaldo | |
Author | Previato, Lucia Mendonça | |
Author | LIma, Leonardo Freire de | |
Author | Decote‑Ricardo, Debora | |
Author | Lima, Celio Geraldo Freire de | |
Access date | 2022-01-23T13:29:28Z | |
Available date | 2022-01-23T13:29:28Z | |
Document date | 2021 | |
Citation | LIMA, Israel Diniz et al. X‑linked immunodeficient (XID) mice exhibit high susceptibility to Cryptococcus gattii infection. Scientific Reports, v. 11, 18397, p. 1 - 11, 2021. | pt_BR |
ISSN | 2045-2322 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/50850 | |
Language | eng | pt_BR |
Publisher | Nature | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Camundongos | pt_BR |
Subject in Portuguese | Alta suscetibilidade | pt_BR |
Subject in Portuguese | Infecção | pt_BR |
Subject in Portuguese | Cryptococcus gattii | pt_BR |
Title | X‑linked immunodeficient (XID) mice exhibit high susceptibility to Cryptococcus gattii infection | pt_BR |
Type | Article | pt_BR |
DOI | 10.1038/s41598-021-97041-9 | |
Abstract | Cryptococcosis is an opportunistic disease caused by the fungus Cryptococcus neoformans and
Cryptococcus gattii. It starts as a pulmonary infection that can spread to other organs, such as the
brain, leading to the most serious occurrence of the disease, meningoencephalitis. The humoral
response has already been described in limiting the progression of cryptococcosis where the B-1 cell
seems to be responsible for producing natural IgM antibodies, crucial for combating fungal infections.
The role of the B-1 cell in C. neoformans infection has been initially described, however the role of the
humoral response of B-1 cells has not yet been evaluated during C. gattii infections. In the present
study we tried to unravel this issue using XID mice, a murine model deficient in the Btk protein which
compromises the development of B-1 lymphocytes. We use the XID mice compared to BALB/c mice
that are sufficient for the B-1 population during C. gattii infection. Our model of chronic lung infection
revealed that XID mice, unlike the sufficient group of B-1, had early mortality with significant weight
loss, in addition to reduced levels of IgM and IgG specific to GXM isolated from the capsule of C.
neoformans. In addition to this, we observed an increased fungal load in the blood and in the brain. We
described an increase in the capsular size of C. gattii and the predominant presence of cytokines with
a Th2 profile was also observed in these animals. Thus, the present study strongly points to a higher
susceptibility of the XID mouse to C. gattii, which suggests that the presence of B-1 cells and anti-GXM
antibodies is fundamental during the control of infection by C. gattii. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Seropédica, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Faculdade de Medicina. Rio de Janeiro, RJ, Brasil.. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal Rural do Rio de Janeiro. Instituto de Veterinária. Seropédica, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Cryptococcus gattii | pt_BR |
Subject | Infection | pt_BR |
Subject | X‑linked immunodeficient (XID | pt_BR |
Subject | Mice | pt_BR |
Subject | High susceptibility | pt_BR |