Author | Pedras, Mariana Junqueira | |
Author | Viana, Luciana de Gouvêa | |
Author | Oliveira, Edward José de | |
Author | Rabello, Ana Lucia Teles | |
Access date | 2022-01-03T15:37:48Z | |
Available date | 2022-01-03T15:37:48Z | |
Document date | 2008 | |
Citation | PEDRAS, Mariana Junqueira et al. Comparative evaluation of direct agglutination test, rK39 and soluble antigen ELISA and IFAT for the diagnosis of visceral leishmaniasis. Transactions of the Royal Society of Tropical Medicine and Hygiene, v. 102, n. 2, p. 172-178, 2008. | pt_BR |
ISSN | 0035-9203 | |
URI | https://www.arca.fiocruz.br/handle/icict/50599 | |
Sponsorship | CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICO | pt_BR |
Language | eng | pt_BR |
Publisher | Royal Society of Tropical Medicine and Hygiene. | pt_BR |
Rights | restricted access | pt_BR |
Title | Comparative evaluation of direct agglutination test, rK39 and soluble antigen ELISA and IFAT for the diagnosis of visceral leishmaniasis | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.trstmh.2007.11.004 | |
Abstract | Five serological tests for the diagnosis of visceral. leishmaniasis (VL) were compared: a direct agglutination test (DAT) based on freeze-dried antigen (DAT-fd); a locally produced DAT (DAT-LPC); an IgG ELISA against rK39 (ELISA-rK39); an IgG ELISA for Leishmania chagasi (ELISA-L. chagasi); and an IgG IFAT against L. chagasi. Serum samples from 88 patients with VL, 20 non-infected individuals and 85 patients with others infectious diseases were evaluated. The sensitivity rates were: DAT-fd, 96.6%; DAT-LPC, 95.5%; ELISA-rK39, 88.6%; ELISA-L. chagasi, 89.8%; and IFAT, 92.0% (P > 0.05). The specificity for the control groups varied from 53.3% to 100%. DAT-fd had the highest efficiency (97.4%), foltowed by DAT-LPC (91.7%) and ELISA-rK39 (90.7%). Our data suggest that DAT-fd, DAT-LPC and ELISA-rK39 are useful tests for the diagnosis of VL and could replace IFAT as the routine diagnostic test in Brazil. (c) 2007 Royal Society of Tropical, Medicine and Hygiene. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisa Clínica. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisa Clínica. Belo Horizonte, MG, Brazil / Universidade Federal de Minas Gerais. Faculdade de Medicina. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisa Clínica. Belo Horizonte, MG, Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisa Clínica. Belo Horizonte, MG, Brazil. | pt_BR |
Subject | Visceral teishmaniasis | pt_BR |
Subject | Diagnosis | pt_BR |
Subject | Serological tests | pt_BR |
Subject | Direct agglutination test | pt_BR |
Subject | rK39 | pt_BR |
Subject | Sensitivity and Specificity | pt_BR |