Author | Silva, Taís Gusmão da | |
Author | Silva, Josefa Carolaine Pereira da | |
Author | Carneiro, Joara Nályda Pereira | |
Author | Amaral, Wanderlei do | |
Author | Deschamps, Cícero | |
Author | Araújo, Jenifer Priscila de | |
Author | Costa, José Galberto Martins da | |
Author | Almeida, Waltécio de Oliveira | |
Author | Silva, Luiz Everson da | |
Author | Coutinho1, Henrique Douglas Melo | |
Author | Ribeiro Filho, Jaime | |
Author | Braga, Maria Flaviana Bezerra Morais | |
Access date | 2021-12-20T18:01:17Z | |
Available date | 2021-12-20T18:01:17Z | |
Document date | 2021 | |
Citation | SILVA, Taís Gusmão da et al. Phytochemical characterization and inhibition of Candida sp. by the essential oil of Baccharis trimera (Less.) DC. Archives of Microbiology, 2021. | pt_BR |
ISSN | 0302-8933 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/50489 | |
Sponsorship | Fundação Cearense
de Apoio ao Desenvolvimento Científico e Tecnológico, FUNCAP for
financial support. | pt_BR |
Language | eng | pt_BR |
Publisher | Springer | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Baccharis | pt_BR |
Subject in Portuguese | Terpenos | pt_BR |
Subject in Portuguese | Óleos essenciais | pt_BR |
Subject in Portuguese | Candida | pt_BR |
Title | Phytochemical characterization and inhibition of Candida sp. by the essential oil of Baccharis trimera (Less.) DC | pt_BR |
Type | Article | pt_BR |
Abstract | This study aimed to investigate the chemical composition and antifungal potential of the essential oil of Baccharis trimera
(Less.) DC. against Candida strains. The half maximal inhibitory concentration (
IC50) was assessed by the microdilution
method using the essential oil at a concentration range of 8192 to 8 μg/mL. The minimum fungicide concentration (MFC)
was determined by subculture in solid medium. The ability of the essential oil to modulate the activity of antifungals was
determined in wells treated simultaneously with the oil at a subinhibitory concentration (MFC/16) and fluconazole (FCZ).
The fungal morphology was analyzed by microscopy. Gas chromatography coupled with mass spectrometry (GC/MS) was
used to identify the chemical composition. The essential oil presented an CI50
of 11.24 and 1.45 μg/mL, which was found to
potentiate the effect of FCZ against Candida albicans. On the other hand, this combined treatment resulted in antagonism
against Candida tropicalis and no evident modulation against Candida krusei was observed. The essential oil significantly
inhibited hyphae growth. However, with a MFC ≥ 16,384 μg/mL, it is assumed that it has a fungistatic action. The antifungal
properties demonstrated in this study might be related to the presence of sesquiterpenes and monoterpenes, and the interaction
between them. In conclusion, Baccharis trimera showed promising anti-Candida effects, in addition to potentiating the
activity of FCZ against Candida albicans, affecting its morphological transition. Therefore, this species constitutes a source
of chemical compounds with the potential to be used in the combat of fungal infections. | pt_BR |
Affilliation | Universidade Regional do Cariri. Department of Biological Chemistry. Crato, CE, Brasil. | pt_BR |
Affilliation | Universidade Regional do Cariri. Department of Biological Chemistry. Crato, CE, Brasil. | pt_BR |
Affilliation | Universidade Regional do Cariri. Department of Biological Chemistry. Crato, CE, Brasil. | pt_BR |
Affilliation | Universidade Federal do Paraná. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Universidade Federal do Paraná. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Universidade Federal do Paraná. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Universidade Regional do Cariri. Department of Biological Chemistry. Crato, CE, Brasil. | pt_BR |
Affilliation | Universidade Regional do Cariri. Department of Biological Chemistry. Crato, CE, Brasil. | pt_BR |
Affilliation | Universidade Federal do Paraná. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Universidade Regional do Cariri. Department of Biological Chemistry. Crato, CE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil. | pt_BR |
Affilliation | Universidade Regional do Cariri. Department of Biological Chemistry. Crato, CE, Brasil. | pt_BR |
Subject | Baccharis trimera · | pt_BR |
Subject | Terpenes · | pt_BR |
Subject | Essential oils · | pt_BR |
Subject | Anti-Candida effect | pt_BR |
e-ISSN | 10.1007/s00203-021-02304-8 | |