Author | Tiburcio, Rafael | |
Author | Melo, Léon Dimitri | |
Author | Nunes, Sara | |
Author | Barbosa, Ana Luísa Augusto | |
Author | Oliveira, Elaine Carvalho de | |
Author | Suarez, Martha | |
Author | Borges, Valéria M. | |
Author | Tavares, Natalia | |
Author | Brodskyn, Claudia Ida | |
Access date | 2021-12-14T17:56:45Z | |
Available date | 2021-12-14T17:56:45Z | |
Document date | 2021 | |
Citation | TIBURCIO, Rafael et al. DC-SIGN Mediates the Interaction Between Neutrophils and Leishmania amazonensis-Infected Dendritic Cells to Promote DC Maturation and Parasite Elimination. Frontiers in Immunology, 2021. | pt_BR |
ISSN | 1664-3224 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/50420 | |
Sponsorship | National Council of Research
(CNPq–Universal) grants 476926/2011-4 for CB and FAPESB
APP0108/2016 for VMB. VMB and CB are senior investigators
from CNPq. The funders had no role in study design, data collection
and analysis, decision to publish, or preparation of the manuscript. | pt_BR |
Language | eng | pt_BR |
Publisher | Frontiers Media | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Leishmaniose | pt_BR |
Subject in Portuguese | Neutrófilos | pt_BR |
Subject in Portuguese | Células dendríticas | pt_BR |
Subject in Portuguese | Monócitos | pt_BR |
Subject in Portuguese | Maturação | pt_BR |
Title | DC-SIGN Mediates the Interaction Between Neutrophils and Leishmania amazonensis-Infected Dendritic Cells to Promote DC Maturation and Parasite Elimination | pt_BR |
Type | Article | pt_BR |
Abstract | Background: Leishmaniasis is a neglected arthropod-borne disease that affects millions
of people worldwide. Successful Leishmania infections require the mitigation of immune
cell functions leading to parasite survival and proliferation. A large body of evidence
highlights the involvement of neutrophils (PMNs) and dendritic cells (DCs) in the
establishment of immunological responses against these parasites. However, few
studies, contemplate to what extent these cells interact synergistically to constrain
Leishmania infection.
Objective: We sought to investigate how PMNs and infected DCs interact in an in vitro
model of Leishmania amazonensis infection.
Material and Methods: Briefly, human PMNs and DCs were purified from the peripheral
blood of healthy donors. Next, PMNs were activated with fibronectin and subsequently
co-cultured with L. amazonensis-infected DCs.
Results: We observed that L. amazonensis-infected DC exhibited lower rates of infection
when co-cultivated with either resting or activated PMNs. Surprisingly, we found that the
release of neutrophil enzymes was not involved in Leishmania killing. Next, we showed
that the interaction between PMNs and infected-DCs was intermediated by DC-SIGN,
further suggesting that parasite elimination occurs in a contact-dependent manner.
Furthermore, we also observed that TNFa and ROS production was dependent on
DC-SIGN-mediated contact, as well as parasite elimination is dependent on TNFa
production in the co-culture. Finally, we observed that direct contact between PMNs
and DCs are required to restore the expression of DC maturation molecules during
L. amazonensis infection. Conclusion: Our findings suggest that the engagement of direct contact between PMNs
and L. amazonensis-infected DC via DC-SIGN is required for the production of
inflammatory mediators with subsequent parasite elimination and DC maturation. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil / Instituto de Nacional de Ciência e Tecnologia. Instituto de Investigação em Imunologia. São Paulo, SP, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Instituto de Nacional de Ciência e Tecnologia. Instituto de Investigação em Imunologia. São Paulo, SP, Brasil. | pt_BR |
Subject | Leishmaniasis | pt_BR |
Subject | Polymorphonuclear cells | pt_BR |
Subject | Monocyte-derived dendritic cells | pt_BR |
Subject | DC-SIGN | pt_BR |
Subject | DC maturation markers | pt_BR |