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https://www.arca.fiocruz.br/handle/icict/50365
LONG NON-CODING RNAS ASSOCIATED WITH RIBOSOMES IN HUMAN ADIPOSE-DERIVED STEM CELLS: FROM RNAS TO MICROPROTEINS
RNA, Long Noncoding
Ribosomes
Open Reading Frames
Microprotein
Protein Biosynthesis
Stem Cells
Ribosomas
Sistemas de Lectura Abierta
Biosíntesis de Proteínas
Células Madre
Ribossomos
Fases de Leitura Aberta
Biossíntese de Proteínas
Células-Tronco
Affilliation
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Regulação da Expressão Gênica. Curitiba, PR, Brasil.
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Biologia Básica de Células Tronco. Curitiba, PR, Brasil.
Abstract
Ribosome profiling reveals the translational dynamics of mRNAs by capturing a ribosomal footprint snapshot. Growing evidence shows that several long non-coding RNAs (lncRNAs) contain small open reading frames (smORFs) that are translated into functional peptides. The difficulty in identifying bona-fide translated smORFs is a constant challenge in experimental and bioinformatics fields due to their unconventional characteristics. This motivated us to isolate human adipose-derived stem cells (hASC) from adipose tissue and perform a ribosome profiling followed by bioinformatics analysis of transcriptome, translatome, and ribosome-protected fragments of lncRNAs. Here, we demonstrated that 222 lncRNAs were associated with the translational machinery in hASC, including the already demonstrated lncRNAs coding microproteins. The ribosomal occupancy of some transcripts was consistent with the translation of smORFs. In conclusion, we were able to identify a subset of 15 lncRNAs containing 35 smORFs that likely encode functional microproteins, including four previously demonstrated smORF-derived microproteins, suggesting a possible dual role of these lncRNAs in hASC self-renewal.
Keywords in Portuguese
MicroproteínaKeywords
lncRNARNA, Long Noncoding
Ribosomes
Open Reading Frames
Microprotein
Protein Biosynthesis
Stem Cells
Keywords in Spanish
ARN Largo no CodificanteRibosomas
Sistemas de Lectura Abierta
Biosíntesis de Proteínas
Células Madre
DeCS
RNA Longo não CodificanteRibossomos
Fases de Leitura Aberta
Biossíntese de Proteínas
Células-Tronco
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