Author | Oliveira, Felipe P. de | |
Author | Rodrigues, Ana Carolina B. da C. | |
Author | Lima, Emilly J. S. P. de | |
Author | Silva, Valdenizia R. | |
Author | Santos, Luciano de S. | |
Author | Anunciação, Talita A. da | |
Author | Nogueira, Mateus L. | |
Author | Soares, Milena Botelho Pereira | |
Author | Dias, Rosane B. | |
Author | Rocha, Clarissa Araujo Gurgel | |
Author | Duvoisin Junior, Sérgio | |
Author | Albuquerque, Patrícia M. | |
Author | Lima, Emerson S. | |
Author | Gonçalves, José F. C. | |
Author | Bataglion, Giovana A. | |
Author | Costa, Emmanoel V. | |
Author | Silva, Felipe M. A. da | |
Author | Koolen, Hector H. F. | |
Author | Bezerra, Daniel Pereira | |
Access date | 2021-12-07T11:17:51Z | |
Available date | 2021-12-07T11:17:51Z | |
Document date | 2021 | |
Citation | OLIVEIRA, Felipe P. de et al. Essential Oil from Bark of Aniba parviflora (MEISN.) MEZ (Lauraceae) Reduces HepG2 Cell Proliferation and Inhibits Tumor Development in a Xenograft Model. Chemistry and Biodiversity, 2021. | pt_BR |
ISSN | 1612-1872 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/50190 | |
Description | Gonçalo Moniz Institute, Oswaldo Cruz Foundation (IGM-FIOCRUZ/BA),
40296-710 Salvador, Bahia, Brazil
or, e-mail: danielpbezerra@gmail.com; daniel.bezerra@fiocruz.br
b Metabolomics and Mass Spectrometry Research Group, Amazonas State University (UEA),
690065-130 Manaus, Amazonas, Brazil
or, e-mail: hectorkoolen@gmail.com; hkoolen@uea.edu.br
c School of Medicine and School of Dentistry, Federal University of Bahia, 40301-155 Salvador, Bahia, Brazil
d Laboratory of Applied Chemistry and Technology, Amazonas State University (UEA),
69050-020 Manaus, Amazonas, Brazil
e Faculty of Pharmacy, Federal University of Amazonas (UFAM),
69077-000 Manaus, Amazonas, Brazil
f Laboratory of Plant Physiology and Biochemistry, National Institute for Amazonian Research (INPA),
69011-970 Manaus, Amazonas, Brazil
g Department of Chemistry, Federal University of Amazonas (UFAM),
69080-900 Manaus, Amazonas, Brazil
h Analytical Center, Multidisciplinary Support Center (CAM), Federal University of Amazonas (UFAM),
69080-900 Manaus, Amazonas, Brazil | pt_BR |
Sponsorship | Conselho Nacional de Desenvolvimento Científico e
Tecnológico (CNPq, Brazil); Coordenação de Aperfeiçoamento
de Pessoal de Nível Superior (CAPES, Brazil,
finance code 001); Fundação de Amparo à Pesquisa do
Estado da Bahia (FAPESB, Brazil); and Fundação de
Amparo à Pesquisa do Estado do Amazonas (FAPEAM,
Brazil). | pt_BR |
Language | eng | pt_BR |
Publisher | Wiley | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Plantas | pt_BR |
Subject in Portuguese | Agentes antitumorais | pt_BR |
Subject in Portuguese | Citotoxicidade | pt_BR |
Subject in Portuguese | Células Hep G2 | pt_BR |
Subject in Portuguese | Lauraceae | pt_BR |
Title | Essential Oil from Bark of Aniba parviflora (MEISN.) MEZ (Lauraceae) Reduces HepG2 Cell Proliferation and Inhibits Tumor Development in a Xenograft Model | pt_BR |
Type | Article | pt_BR |
DOI | 10.1002/cbdv.202000938 | |
Abstract | Aniba parviflora (MEISN.) MEZ (Lauraceae) is an aromatic plant of the Amazon rainforest, which has a
tremendous commercial value in the perfumery industry; it is popularly used as flavoring sachets and aromatic
baths. In Brazilian folk medicine, A. parviflora is used to treat victims of snakebites. Herein, we analyzed the
chemical composition of A. parviflora bark essential oil (EO) and its effect on the growth of human hepatocellular
carcinoma HepG2 cells in vitro and in vivo. EO was obtained by hydrodistillation and characterized by GC-MS and
GC-FID. The main constituents of EO were linalool (16.3:3.15), α-humulene (14.5:2.41 %), δ-cadinene (10.2:
1.09 %), α-copaene (9.51:1.12%) and germacrene B (7.58:2.15 %). Initially, EO’s cytotoxic effect was evaluated
against five cancer cell lines (HepG2, MCF-7, HCT116, HL-60 and B16-F10) and one non-cancerous one (MRC-5),
using the Alamar blue method after 72 h of treatment. The calculated IC50 values were 9.05, 22.04, >50, 15.36,
17.57, and 30.46 μg/mL, respectively. The best selectivity was for HepG2 cells with a selective index of 3.4. DNA
Fragmentation and cell cycle distribution were quantified in HepG2 cells by flow cytometry after a treatment
period of 24 and 48 h. The effect of EO on tumor development in vivo was evaluated in a xenograft model using
C.B-17 SCID mice engrafted with HepG2 cells. In vivo tumor growth inhibition of HepG2 xenograft at the doses of
40 and 80 mg/kg were 12.1 and 62.4 %, respectively. | pt_BR |
Affilliation | "Múltipla ver em Notas" | pt_BR |
Subject | Aniba parviflora | pt_BR |
Subject | Antitumor agents | pt_BR |
Subject | Cytotoxicity | pt_BR |
Subject | HepG2 | pt_BR |
Subject | Lauraceae | pt_BR |