Author | Marcos, Caroline Maria | |
Author | Oliveira, Haroldo Cesar de | |
Author | Assato, Patrícia Akemi | |
Author | Castelli, Rafael Fernando | |
Author | Fusco-Almeida, Ana Marisa | |
Author | Mendes-Giannini, Maria José Soares | |
Access date | 2021-11-25T16:47:59Z | |
Available date | 2021-11-25T16:47:59Z | |
Document date | 2021 | |
Citation | MARCOS, Caroline Maria et al. Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis. J. Fungi, V.7, n. 852, p. 1–22, 2021. | pt_BR |
ISSN | 2309-608X | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/50063 | |
Language | por | pt_BR |
Publisher | MDPI | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Drk1 | pt_BR |
Title | Drk1, a dimorphism histidine kinase, contributes to morphology, virulence, and stress adaptation in Paracoccidioides brasiliensis | pt_BR |
Type | Article | pt_BR |
DOI | 10.3390/jof7100852 | |
Abstract | P. brasiliensis is a thermally dimorphic fungus belonging to Paracoccidioides complex, causative of a systemic, endemic mycosis limited to Latin American countries. Signal transduction pathways related to important aspects as surviving, proliferation according to the biological niches are linked to the fungal pathogenicity in many species, but its elucidation in P. brasiliensis remains poorly explored. As Drk1, a hybrid histidine kinase, plays regulators functions in other dimorphic fungi species, mainly in dimorphism and virulence, here we investigated its importance in P. brasilensis. We, therefore generated the respective recombinant protein, anti-PbDrk1 polyclonal antibody and a silenced strain. The Drk1 protein shows a random distribution including cell wall location that change its pattern during osmotic stress condition; moreover the P. brasiliensis treatment
with anti-PbDrk1 antibody, which does not modify the fungus’s viability, resulted in decreased virulence in G. mellonella model and reduced interaction with pneumocytes. Down-regulating PbDRK1 yielded phenotypic alterations such as yeast cells with more elongated morphology, virulence attenuation in G. mellonella infection model, lower amount of chitin content, increased resistance to osmotic and cell wall stresses, and also caspofungin, and finally increased sensitivity to itraconazole. These observations highlight the importance of PbDrk1 to P. brasiliensis virulence, stress adaptation, morphology, and cell wall organization, and therefore it an interesting target that could help develop new antifungals | pt_BR |
Affilliation | Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas, Araraquara, SP, Brasil. | pt_BR |
Affilliation | Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas, Araraquara, SP, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas, Araraquara, SP, Brasil. / Universidade Estadual Paulista. Faculdade de Ciências Agronômicas. Laboratório Central Multiusuários. Botucatu, SP, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa de Pós-Graduação em Biologia Parasitária. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas, Araraquara, SP, Brasil. | pt_BR |
Affilliation | Universidade Estadual Paulista. Faculdade de Ciências Farmacêuticas, Araraquara, SP, Brasil. | pt_BR |
Subject | Histidine Kinase | pt_BR |
Subject | Paracoccidioides brasiliensis | pt_BR |
Subject | Broadly Neutralizing Antibodies | pt_BR |
Subject | RNA Silencing | pt_BR |
Subject | Cell Wall | pt_BR |
Subject | Virulence | pt_BR |
Subject in Spanish | Histidina Quinasa | pt_BR |
Subject in Spanish | Paracoccidioides | pt_BR |
Subject in Spanish | Anticuerpos ampliamente neutralizantes | pt_BR |
Subject in Spanish | Interferencia de ARN | pt_BR |
Subject in Spanish | Pared Celular | pt_BR |
Subject in Spanish | Virulencia | pt_BR |
Subject in French | Histidine kinase | pt_BR |
Subject in French | Paracoccidioides | pt_BR |
Subject in French | Interférence par ARN | pt_BR |
Subject in French | Paroi cellulaire | pt_BR |
DeCS | Histidina Quinase | pt_BR |
DeCS | Paracoccidioides | pt_BR |
DeCS | Anticorpos Amplamente Neutralizantes | pt_BR |
DeCS | Interferência de RNA | pt_BR |
DeCS | Parede Celular | pt_BR |
DeCS | Virulência | pt_BR |