Author | Conzentino, Marcelo S. | |
Author | Forchhammer, Karl | |
Author | Souza, Emanuel Maltempi de | |
Author | Pedrosa, Fábio O. | |
Author | Nogueira, Meri B. | |
Author | Raboni, Sônia M. | |
Author | Rego, Fabiane G. M. | |
Author | Zanette, Dalila Lucíola | |
Author | Aoki, Mateus Nóbrega | |
Author | Nardin, Jeanine M. | |
Author | Fornazari, Bruna | |
Author | Morales, Hugo M. P. | |
Author | Celedon, Paola Alejandra Fiorani | |
Author | Lima, Carla V. P. | |
Author | Mattar, Sibelle B. | |
Author | Lin, Vanessa H. | |
Author | Morello, Luis Gustavo | |
Author | Marchini, Fabrício Klerynton | |
Author | Reis, Rodrigo A. | |
Author | Huergo, Luciano F. | |
Access date | 2021-11-12T15:24:25Z | |
Available date | 2021-11-12T15:24:25Z | |
Document date | 2021 | |
Citation | CONZENTINO, Marcelo S. et al. Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS‑CoV‑2 seroconversion. Brazilian Journal of Microbiology. p. 1–5, 2021. | pt_BR |
ISSN | 1678-4405 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/49817 | |
Language | por | pt_BR |
Publisher | Sociedade Brasileira de Microbiologia | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | SARS-CoV-2 | pt_BR |
Subject in Portuguese | COVID-19 | pt_BR |
Subject in Portuguese | ELISA | pt_BR |
Subject in Portuguese | Proteínas do Nucleocapsídeo | pt_BR |
Title | Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS‑CoV‑2 seroconversion | pt_BR |
Type | Article | pt_BR |
DOI | 10.1007/s42770-021-00556-6 | |
Abstract | Serological assays are important tools to identify previous exposure to SARS-CoV-2, helping to track COVID-19 cases and determine the level of humoral response to SARS-CoV-2 infections and/or immunization to future vaccines. Here, the SARS-CoV-2 nucleocapsid protein was expressed in Escherichia coli and purifed to homogeneity and high yield using a single chromatography step. The purifed SARS-CoV-2 nucleocapsid protein was used to develop an indirect enzyme-linked immunosorbent assay for the identifcation of human SARS-CoV-2 seroconverts. The assay sensitivity and specifcity were determined analyzing sera from 140 RT-qPCR-confrmed COVID-19 cases and 210 pre-pandemic controls. The assay operated with 90% sensitivity and 98% specifcity; identical accuracies were obtained in head-to-head comparison with a commercial ELISA kit. Antigen-coated plates were stable for up to 3 months at 4 °C. The ELISA method described is ready
for mass production and will be an additional tool to track COVID-19 cases. | pt_BR |
Affilliation | Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil. | pt_BR |
Affilliation | Interfakultäres Institut für Mikrobiologie und Infektionsmedizin der Eberhard-Karls. Universität Tübingen. Tübingen, Germany. | pt_BR |
Affilliation | Universidade Federal do Paraná. Departamento de Bioquímica e Biologia Molecular. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Universidade Federal do Paraná. Departamento de Bioquímica e Biologia Molecular. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Universidade Federal do Paraná. Hospital das Clínicas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Universidade Federal do Paraná. Hospital das Clínicas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Universidade Federal do Paraná. Programa de Pós-Graduação em Ciências Farmacêuticas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Hospital Erasto Gaertner. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Hospital Erasto Gaertner. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Hospital Erasto Gaertner. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil. | pt_BR |
Affilliation | Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil. | pt_BR |
Subject | Immunologic Tests | pt_BR |
Subject | Enzyme-Linked Immunosorbent Assay | pt_BR |
Subject | Nucleocapsid Proteins | pt_BR |
Subject in Spanish | Pruebas Inmunológicas | pt_BR |
Subject in Spanish | Ensayo de Inmunoadsorción Enzimática | pt_BR |
Subject in Spanish | Proteínas de la Nucleocápside | pt_BR |
Subject in French | Tests immunologiques | pt_BR |
Subject in French | Test ELISA | pt_BR |
Subject in French | Protéines nucléocapside | pt_BR |
DeCS | Testes Imunológicos | pt_BR |
DeCS | Ensaio de Imunoadsorção Enzimática | pt_BR |