Author | Jácome, Fernanda Cunha | |
Author | Caldas, Gabriela Cardoso | |
Author | Rasinhas, Arthur da Costa | |
Author | Almeida, Ana Luisa Teixeira de | |
Author | Souza, Daniel Dias Coutinho de | |
Author | Paulino, Amanda Carlos | |
Author | Silva, Marcos Alexandre Nunes da | |
Author | Barth, Ortrud Monika | |
Author | Santos, Flávia Barreto dos | |
Author | Vieira, Debora Ferreira Barreto | |
Access date | 2021-09-14T11:12:26Z | |
Available date | 2021-09-14T11:12:26Z | |
Document date | 2021 | |
Citation | JÁCOME, Fernanda Cunha et al. Brazilian Dengue Virus Type 2-Associated Renal Involvement in a Murine Model: Outcomes after Infection by Two Lineages of the Asian/American Genotype. Pathogens, v. 10, 1084, 20 p, 2021. | pt_BR |
ISSN | 2076-7817 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/49071 | |
Language | eng | pt_BR |
Publisher | MDPI | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Dengue 2 | pt_BR |
Subject in Portuguese | Linhagens asiáticas / americanas | pt_BR |
Subject in Portuguese | BALB/c mice | pt_BR |
Subject in Portuguese | Rim | pt_BR |
Subject in Portuguese | Histopatologia e microscopia eletrônica de transmissão | pt_BR |
Title | Brazilian Dengue Virus Type 2-Associated Renal Involvement in a Murine Model: Outcomes after Infection by Two Lineages of the Asian/American Genotype | pt_BR |
Type | Article | pt_BR |
DOI | 10.3390/pathogens10091084 | |
Abstract | Dengue virus type 2 (DENV-2) is, traditionally, the most studied serotype due to its
association with explosive outbreaks and severe cases. In Brazil, almost 20 years after the first
introduction in the 1990s, a new lineage (Lineage II) of the DENV-2 Asian/American genotype
emerged and caused an epidemic with severe cases and hospitalizations. Severe dengue includes
multiple organ failure, and renal involvement can be potentially related to increased mortality. In
order to better understand the role of DENV infection in renal injury, here we aimed to investigate the
outcomes of infection with two distinct lineages of DENV-2 Asian/American genotype in the kidney
of a murine model. BALB/c mice were infected with Lineages I and II and tissues were submitted to
histopathology, immunohistochemistry, histomorphometry and ultrastructural analysis. Blood urea
nitrogen (BUN) was detected in blood sample accessed by cardiac puncture. A tendency in kidney
weight increase was observed in mice infected with both lineages, but urea levels, on average, were
increased only in mice infected with Lineage II. The DENV antigen was detected in the tissue of
mice infected with Lineage II and morphological changes were similar to those observed in human
dengue cases. Furthermore, the parameters such as organ weight, urea levels and morphometric
analysis, showed significant differences between the two lineages in the infected BALB/c, which was
demonstrated to be a suitable experimental model for dengue pathophysiology studies in kidneys. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Dengue 2 | pt_BR |
Subject | Asian/American lineages | pt_BR |
Subject | BALB/c mice | pt_BR |
Subject | Kidney | pt_BR |
Subject | Histopathology and transmission electron microscopy | pt_BR |