Please use this identifier to cite or link to this item:
https://www.arca.fiocruz.br/handle/icict/48784
Type
ArticleCopyright
Open access
Collections
- INI - Artigos de Periódicos [3392]
- IOC - Artigos de Periódicos [12488]
Metadata
Show full item record
AEDES ALBOPICTUS DIVERSITY AND RELATIONSHIPS IN SOUTH-WESTERN EUROPE AND BRAZIL BY RDNA/ MTDNA AND PHENOTYPIC ANALYSES: ITS-2, A USEFUL MARKER FOR SPREAD STUDIES
Haplotipagem molecular
Clonagem
Sequenciamento
Morfometria geométrica de asa
Vetor de doença
Brasil
Sudoeste da Europa
Molecular haplotyping
rDNA 5.8S-ITS-2
mtDNA cox1
Cloning
Sequencing
Wing geometric morphometry
Disease vector
South-western Europe
Brazil
Author
Affilliation
Universidad de Valencia. Facultad de Farmacia. Departamento de Parasitologia. Valencia, Spain.
Universidad de Valencia. Facultad de Farmacia. Departamento de Parasitologia. Valencia, Spain.
Universidad de Valencia. Facultad de Farmacia. Departamento de Parasitologia. Valencia, Spain.
Universidad de Valencia. Facultad de Farmacia. Departamento de Parasitologia. Valencia, Spain.
Universidad de Valencia. Facultad de Farmacia. Departamento de Parasitologia. Valencia, Spain / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica e Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.
Instituto de Investigación Agroalimentario de Aragón IA2, Facultad de Veterinaria, Universidad de Zaragoza. Zaragoza, Spain.
Universidad de las Islas Baleares. Departamento de Biología. Grupo de Zoología Aplicada y de La Conservación. Palma de Mallorca, Spain.
Instituto de Investigación Agroalimentario de Aragón IA2, Facultad de Veterinaria, Universidad de Zaragoza. Zaragoza, Spain.
Universidad de Valencia. Facultad de Farmacia. Departamento de Parasitologia. Valencia, Spain.
Universidad de Valencia. Facultad de Farmacia. Departamento de Parasitologia. Valencia, Spain.
Universidad de Valencia. Facultad de Farmacia. Departamento de Parasitologia. Valencia, Spain.
Universidad de Valencia. Facultad de Farmacia. Departamento de Parasitologia. Valencia, Spain.
Universidad de Valencia. Facultad de Farmacia. Departamento de Parasitologia. Valencia, Spain.
Universidad de Valencia. Facultad de Farmacia. Departamento de Parasitologia. Valencia, Spain / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica e Vigilância em Leishmanioses. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.
Instituto de Investigación Agroalimentario de Aragón IA2, Facultad de Veterinaria, Universidad de Zaragoza. Zaragoza, Spain.
Universidad de las Islas Baleares. Departamento de Biología. Grupo de Zoología Aplicada y de La Conservación. Palma de Mallorca, Spain.
Instituto de Investigación Agroalimentario de Aragón IA2, Facultad de Veterinaria, Universidad de Zaragoza. Zaragoza, Spain.
Universidad de Valencia. Facultad de Farmacia. Departamento de Parasitologia. Valencia, Spain.
Universidad de Valencia. Facultad de Farmacia. Departamento de Parasitologia. Valencia, Spain.
Abstract
Background: Aedes albopictus is a very invasive mosquito, which has recently colonized tropical and temperate regions worldwide. Of concern is its role in the spread of emerging or re-emerging mosquito-borne diseases. Ae. albopictus from south-western Europe and Brazil were studied to infer genetic and phenetic diversity at intra-individ‑ ual, intra-population and inter-population levels, and to analyse its spread. Methods: Genotyping was made by rDNA 5.8S-ITS-2 and mtDNA cox1 sequencing to assess haplotype and nucleo‑ tide diversity, genetic distances and phylogenetic networks. Male and female phenotyping included combined landmark-and outlined-based geometric morphometrics of wing size and shape. Results: Specimens from seven populations from Spain, France and Brazil provided 12 cox1 and 162 5.8S-ITS-2 haplotypes, with great genetic variability diference between both markers (0.9% vs 31.2%). Five cox1 haplotypes were shared with other countries, mainly Italy, USA and China, but none was shared between Europe and Brazil. The 5.8S-ITS-2 showed 2–7 intra-individual (mean 4.7) and 16–34 intra-/inter-population haplotypes (24.7), including haplotypes shared between Spain, France and Brazil. A 4.3% of ITS-2 haplotypes were shared, mainly with Italy, USA and Thailand, evidencing worldwide spread and introductions from areas where recent outbreaks of Ae. albopictus transmitted pathogens occurred. Wing size showed sex diferences. Wing shape distinguished between Brazilian and European specimens. Both genetic and morphometric markers showed diferences between insular Spain and continental Spain, France and Brazil. Conclusions: ITS-2 proves to be a useful marker to assess Ae. albopictus spread, providing pronouncedly more information than cox1, including intra-individual, intra-population and inter-population levels, furnishing a complete overview of the evolutionary exchanges followed by this mosquito. Wing morphometry proves to be a useful pheno‑ typing marker, allowing to distinguish diferent populations at the level of both male and female specimens. Results indicate the need for periodic surveillance monitorings to verify that no Ae. albopictus with high virus transmission capacity is introduced into Europe.
Keywords in Portuguese
Aedes albopictusHaplotipagem molecular
Clonagem
Sequenciamento
Morfometria geométrica de asa
Vetor de doença
Brasil
Sudoeste da Europa
Keywords
Aedes albopictusMolecular haplotyping
rDNA 5.8S-ITS-2
mtDNA cox1
Cloning
Sequencing
Wing geometric morphometry
Disease vector
South-western Europe
Brazil
Share