Author | Palka, Ana Paula Gori | |
Author | Matos, Tatiana Reichert Assunção de | |
Author | Souza, Claudemir de | |
Author | Eugênio, Danilo Santos | |
Author | Krieger, Marco Aurélio | |
Author | Fragoso, Stenio Perdigão | |
Author | Pavoni, Daniela Parada | |
Access date | 2021-08-11T17:40:59Z | |
Available date | 2021-08-11T17:40:59Z | |
Document date | 2021 | |
Citation | PALKA, Ana Paula Gori et al. Assessing the antigenicity of different VP3 regions of infectious bursal disease virus in chickens from South Brazil. BMC Veterinary Research, v. 17, n. 259, p. 1–9, 2021. | pt_BR |
ISSN | 1746-6148 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/48580 | |
Language | por | pt_BR |
Publisher | BMC | pt_BR |
Rights | open access | pt_BR |
Title | Assessing the antigenicity of different VP3 regions of infectious bursal disease virus in chickens from South Brazil | pt_BR |
Type | Article | pt_BR |
DOI | 10.1186/s12917-021-02956-0 | |
Abstract | Infectious bursal disease (IBD), also known as Gumboro disease, is a viral infection that causes mortality and immunosuppression in chickens (Gallus gallus). VP2 and VP3 are the major structural viral capsid components and are the most immunogenic proteins of IBD virus (IBDV). Reliable diagnostic tests using VP2 and VP3 produced in heterologous systems are important tools to control this infection. One advantage of an IBD diagnostic based on VP3, over those that use VP2, is that VP3 has linear epitopes, enabling its production in bacteria. We tested the suitability of recombinant VP3 (rVP3) as a diagnostic reagent in an enzyme-linked immunosorbent assay (ELISA). Compared with a commercial test, rVP3 ELISA showed high sensitivity and specificity as a diagnostic tool for vaccinated animals. In addition, rVP3, but not the commercial ELISA, was able to detect antibodies in nonvaccinated chickens, probably developed against circulating IBDV strains. It was possible the assessment of VP3 regions antigenicity using chicken antisera. The full-length recombinant VP3 can be used to assess post vaccination immunological status of chickens and its production is feasible and inexpensive. The evaluation of VP3 regions as candidates for general use in the diagnosis of IBD in chickens should be conducted with caution. Our work was the first to identify several regions of VP3 recognized by chicken antibodies | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Instituto de Tecnologia do Paraná. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Programa de Pós-graduação em Biociências e Biotecnologia. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Programa de Pós-graduação em Biociências e Biotecnologia. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Programa de Pós-graduação em Biociências e Biotecnologia. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Universidade Federal do Paraná. Programa de Pós-Graduação em Biologia Celular e Molecular. Curitiba, PR, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Programa de Pós-graduação em Biociências e Biotecnologia. Curitiba, PR, Brasil. | pt_BR |
Subject | Infectious bursal disease virus | pt_BR |
Subject | Gumboro disease | pt_BR |
Subject | VP3 | pt_BR |
Subject | Viral Structural Proteins | pt_BR |
Subject | Antigenic regions | pt_BR |
Subject | Immunodominant Epitopes | pt_BR |
Subject | Enzyme-Linked Immunosorbent Assay | pt_BR |
Subject in Spanish | Virus de la Enfermedad Infecciosa de la Bolsa | pt_BR |
Subject in Spanish | Proteínas Estructurales Virales | pt_BR |
Subject in Spanish | Epítopos Inmunodominantes | pt_BR |
Subject in Spanish | Ensayo de Inmunoadsorción Enzimática | pt_BR |
Subject in French | Virus de la bursite infectieuse | pt_BR |
Subject in French | Protéines virales structurales | pt_BR |
Subject in French | Épitopes immunodominants | pt_BR |
Subject in French | Test ELISA | pt_BR |
DeCS | Vírus da Doença Infecciosa da Bursa | pt_BR |
DeCS | Proteínas Estruturais Virais | pt_BR |
DeCS | Epitopos Imunodominantes | pt_BR |
DeCS | Ensaio de Imunoadsorção Enzimática | pt_BR |