Author | Oliveira, Valéria da Costa | |
Author | Mendes Junior, Artur Augusto Velho | |
Author | Ferreira, Luiz Claudio | |
Author | Calvet, Tatiana Machado Quinates | |
Author | Santos, Shanna Araujo dos | |
Author | Figueiredo, Fabiano Borges | |
Author | Campos, Monique Paiva | |
Author | Rodrigues, Francisco das Chagas de Carvalho | |
Author | Oliveira, Raquel de Vasconcellos Carvalhaes de | |
Author | Lemos, Elba Regina Sampaio de | |
Author | Rozental, Tatiana | |
Author | Silva, Raphael Gomes da | |
Author | Amendoeira, Maria Regina Reis | |
Author | Teles-de-Freitas, Rayane | |
Author | Bruno, Rafaela Vieira | |
Author | Morgado, Fernanda Nazaré | |
Author | Miranda, Luciana de Freitas Campos | |
Author | Menezes, Rodrigo Caldas | |
Access date | 2021-04-20T20:09:55Z | |
Available date | 2021-04-20T20:09:55Z | |
Document date | 2021 | |
Citation | OLIVEIRA, Valéria da Costa et al. Frequency of co-seropositivities for certain pathogens and their relationship with clinical and histopathological changes and parasite load in dogs infected with Leishmania infantum. Plos One, v.16 n. 3, p. 1–20, 2021 | pt_BR |
ISSN | 1932-6203 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/46792 | |
Language | por | pt_BR |
Publisher | Nature Research | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Leishmaniose | pt_BR |
Subject in Portuguese | Toxoplasma gondii | pt_BR |
Subject in Portuguese | Ehrlichia canis | pt_BR |
Subject in Portuguese | Anaplasma | pt_BR |
Title | Frequency of co-seropositivities for certain pathogens and their relationship with clinical and histopathological changes and parasite load in dogs infected with Leishmania infantum | pt_BR |
Type | Article | pt_BR |
DOI | 10.1371/ journal.pone.0247560 | |
Abstract | In canine leishmaniosis caused by the protozoan Leishmania infantum, little is known about how co-infections with or co-seropositivities for other pathogens can influence aggravation of this disease. Therefore, the objectives of this study were to evaluate the frequency of coinfections with or co-seropositivities for certain pathogens in dogs seropositive for L. infantum and their relationship with clinical signs, histological changes and L. infantum load. Sixty-six L. infantum-seropositive dogs were submitted to clinical examination, collection of blood and bone marrow, culling, and necropsy. Antibodies against Anaplasma spp., Borrelia burgdorferi sensu lato, Ehrlichia spp. and Toxoplasma gondii and Dirofilaria immitis antigens were investigated in serum. Samples from different tissues were submitted to histopathology and immunohistochemistry for the detection of Leishmania spp. and T. gondii. Quantitative real-time PCR was used to assess the L. infantum load in spleen samples. For detection of Coxiella burnetii, conventional PCR and nested PCR were performed using
bone marrow samples. All 66 dogs tested positive for L. infantum by qPCR and/or culture. Fifty dogs (76%) were co-seropositive for at least one pathogen: T. gondii (59%), Ehrlichia spp., (41%), and Anaplasma spp. (18%). Clinical signs were observed in 15 (94%) dogs monoinfected with L. infantum and in 45 (90%) dogs co-seropositive for certain pathogens. The L. infantum load in spleen and skin did not differ significantly between monoinfected and co-seropositive dogs. The number of inflammatory cells was higher in the spleen, lung and mammary gland of co-seropositive dogs and in the mitral valve of monoinfected dogs. These results suggest that dogs infected with L. infantum and co-seropositive for certain pathogens are common in the region studied. However, co-seropositivities for certain pathogens did not aggravate clinical signs or L. infantum load, although they were associated with a more intense inflammatory reaction in some organs. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Leishmania infantum | pt_BR |
Subject | Coinfection | pt_BR |
Subject | Dogs | pt_BR |
Subject | Virulence | pt_BR |
Subject in Spanish | Coinfección | pt_BR |
Subject in Spanish | Perros | pt_BR |
Subject in Spanish | Virulencia | pt_BR |
Subject in French | Co-infection | pt_BR |
Subject in French | Chiens | pt_BR |
DeCS | Leishmania infantum | pt_BR |
DeCS | Coinfecção | pt_BR |
DeCS | Cães | pt_BR |
DeCS | Patogenicidade | pt_BR |