Author | Valladares, Vanessa | |
Author | Pasquini, Célio | |
Author | Thiengo, Silvana C. | |
Author | Fernandez, Monica A. | |
Author | Silva, Clélia C. Mello | |
Access date | 2021-04-20T13:38:05Z | |
Available date | 2021-04-20T13:38:05Z | |
Document date | 2021 | |
Citation | VALLADARES, Vanessa et al. Field Application of NIR Spectroscopy for the Discrimination of the Biomphalaria Species That Are Intermediate Hosts of Schistosoma mansoni in Brazil. Frontiers in Public Health, v. 9, Article 636206, p. 1-8, Mar. 2021. | pt_BR |
ISSN | 2296-2565 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/46785 | |
Language | eng | pt_BR |
Publisher | Frontiers Media | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Espectroscopia infravermelha próxima | pt_BR |
Subject in Portuguese | Técnica inovadora | pt_BR |
Subject in Portuguese | Molusco | pt_BR |
Subject in Portuguese | Caracóis de água doce | pt_BR |
Subject in Portuguese | Fenótipo químico | pt_BR |
Subject in Portuguese | Esquistossomose | pt_BR |
Title | Field Application of NIR Spectroscopy for the Discrimination of the Biomphalaria Species That Are Intermediate Hosts of Schistosoma mansoni in Brazil | pt_BR |
Type | Article | pt_BR |
DOI | 10.3389/fpubh.2021.636206 | |
Abstract | Near Infrared Spectroscopy (NIRS) is a spectroscopic technique that evaluates the
vibrational energy levels of the chemical bonds of molecules within a wavelength range
of 750–2,500 nm. This simple method acquires spectra that provide qualitative and
quantitative data on the chemical components of the biomass of living organisms
through the interaction between the electromagnetic waves and the sample. NIRS is an
innovative, rapid, and non-destructive technique that can contribute to the differentiation
of species based on their chemical phenotypes. Chemical profiles were obtained by
NIRS from three snail species (Biomphalaria glabrata, Biomphalaria straminea, and
Biomphalaria tenagophila) that are intermediate hosts of Schistosoma mansoni in Brazil.
The correct identification of these species is important from an epidemiological viewpoint,
given that each species has distinct biological and physiological characteristics. The
present study aimed to develop a chemometric model for the interspecific and intra specific classification of the three species, focusing on laboratory and field populations.
The data were obtained from 271 live animals, including 150 snails recently collected from
the field, with the remainder being raised in the laboratory. Populations were sampled at
three localities in the Brazilian state of Rio de Janeiro, in the municipalities of Sumidouro
(B. glabrata) and Paracambi (B. straminea), and the borough of Jacarepaguá in the Rio
de Janeiro city (B. tenagophila). The chemometric analysis was run in the Unscrambler®
software. The intra-specific classification of the field and laboratory populations obtained
accuracy rates of 72.5% (B. tenagophila), 77.5% (B. straminea), and 85.0% (B. glabrata).
The interspecific differentiation had a hit rate of 75% for the field populations and 80%
for the laboratory populations. The results indicate chemical and metabolic differences
between populations of the same species from the field and the laboratory. The chemical
phenotype, which is closely related to the metabolic profile of the snails, varied between environments. Overall, the NIRS technique proved to be a potentially valuable tool for
medical malacology, enabling the systematic discrimination of the Biomphalaria snails
that are the intermediate hosts of S. mansoni in Brazil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Monitoramento e Prevenção da Saúde Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Estadual de Campinas. Instituto de Química. Campinas, SP, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Malacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Malacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Monitoramento e Prevenção da Saúde Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Near infrared spectroscopy | pt_BR |
Subject | Innovative technique | pt_BR |
Subject | Mollusk | pt_BR |
Subject | Freshwater snails | pt_BR |
Subject | Chemical phenotype | pt_BR |
Subject | Schistosomiasis | pt_BR |