Author | Teixeira, Carlos Graeff | |
Author | Favero, Vivian | |
Author | Pascoal, Vanessa Fey | |
Author | Souza, Renata Perotto de | |
Author | Rigo, Francine de Vargas | |
Author | Agnese, Luize Hoffmann Dall | |
Author | Bezerra, Fernando Schemelzer Moraes | |
Author | Coelho, Paulo Marcos Zech | |
Author | Enk, Martin Johannes | |
Author | Favre, Tereza Cristina | |
Author | Katz, Naftale | |
Author | Oliveira, Ricardo Riccio | |
Author | Reis, Mitermayer Galvão dos | |
Author | Pieri, Otavio Sarmento | |
Access date | 2021-04-04T18:59:02Z | |
Available date | 2021-04-04T18:59:02Z | |
Document date | 2021 | |
Citation | TEIXEIRA, Carlos Graeff et al. Low specificity of point-of-care circulating cathodic antigen (POC–CCA) diagnostic test in a non-endemic area for schistosomiasis mansoni in Brazil. Acta Tropica, v. 217, p. 1-7, 2021. | pt_BR |
ISSN | 0001-706X | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/46495 | |
Language | eng | pt_BR |
Publisher | Elsevier | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | Antígeno catódico circulante (POC-CCA) | pt_BR |
Subject in Portuguese | Helmintex | pt_BR |
Subject in Portuguese | Kato-Katz | pt_BR |
Subject in Portuguese | Brasil | pt_BR |
Subject in Portuguese | Schistosoma mansoni | pt_BR |
Title | Low specificity of point-of-care circulating cathodic antigen (POC–CCA) diagnostic test in a non-endemic area for schistosomiasis mansoni in Brazil | pt_BR |
Type | Article | pt_BR |
DOI | 10.1016/j.actatropica.2021.105863 | |
Abstract | A point-of-care test for detecting schistosome circulating cathodic antigen in urine (POC–CCA) has been proposed
for mapping infection and defining prevalence thresholds for mass drug administration (MDA). However,
there is increasing evidence that POC–CCA may yield false-positive results, which requires rigorous specificity
evaluation in non-endemic areas. POC–CCA was applied in an area known to be free from infection and devoid
of any condition for schistosomiasis transmission as part of a multicentre study to evaluate the performance of
POC–CCA in Brazil’s low or potentially endemic settings. Besides POC–CCA detection in urine, a search for eggs
in stool was performed by Kato-Katz (KK) and Helmintex (HTX) methods. One-hundred-and-seventy-four participants
returned urine samples, 140 of which delivered stool samples. All these were HTX-negative for Schistosoma
mansoni, and all 118 tested with KK were negative for both S. mansoni and soil-transmitted helminths.
POC–CCA results from freshly collected urine yielded a specificity of 62.1% (95% CI: 53.6% - 70.2%), taking
trace outcomes as positive according to the manufacturer’s instructions. Retesting urine from the 140 HTXnegatives
after one-year storage at -20 ◦C with two new POC–CCA batches simultaneously yielded significantly
different specificities (34.3%; 95%CI: 26.5% – 42.8% and 75.0%; 95% CI: 67.0% - 81.9%). These two
batches had a weak agreement (Cohen’s kappa: 0.56; 95%CI: 0.44–0.68) among the 174 urine samples retested.
At present, POC–CCA cannot be recommended either as a cut-off point for MDA or a reliable diagnostic tool for
treatment of the infection carriers (selective chemotherapy) in low endemic areas and at final stages of transmission
interruption. Manufacturers should be required to optimize production standardization and to assure
quality and reproducibility of the test. Extended rigorous performance evaluations by different users from
different regions are needed before POC–CCA is widely recommended. | pt_BR |
Affilliation | Universidade Federal do Espírito Santo. Centro de Ciências da Saúde. Unidade de Doenças Infecciosas. Vitória, ES, Brasill / Pontifícia Universidade Católica do Rio Grande do Sul. Escola de Ciências. Grupo de Pesquisa em Parasitologia Biomédica. Porto Alegre, RS, Brasil. | pt_BR |
Affilliation | Pontifícia Universidade Católica do Rio Grande do Sul. Escola de Ciências. Grupo de Pesquisa em Parasitologia Biomédica. Porto Alegre, RS, Brasil. | pt_BR |
Affilliation | Pontifícia Universidade Católica do Rio Grande do Sul. Escola de Ciências. Grupo de Pesquisa em Parasitologia Biomédica. Porto Alegre, RS, Brasil. | pt_BR |
Affilliation | Pontifícia Universidade Católica do Rio Grande do Sul. Escola de Ciências. Grupo de Pesquisa em Parasitologia Biomédica. Porto Alegre, RS, Brasil. | pt_BR |
Affilliation | Pontifícia Universidade Católica do Rio Grande do Sul. Escola de Ciências. Grupo de Pesquisa em Parasitologia Biomédica. Porto Alegre, RS, Brasil. | pt_BR |
Affilliation | Pontifícia Universidade Católica do Rio Grande do Sul. Escola de Ciências. Grupo de Pesquisa em Parasitologia Biomédica. Porto Alegre, RS, Brasil. | pt_BR |
Affilliation | Universidade Federal do Ceará. Departamento de Análises Clínicas e Toxicológicas. Fortaleza, CE, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Reneé Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Instituto Evandro Chagas. Ananindeua, Pará. Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Reneé Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Muniz. Salvador, BA, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Gonçalo Muniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, Brasil / Department of Epidemiology of Microbial Diseases, School of Public Health, Yale University, New Haven, CT, USA, | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | Schistosoma mansoni | pt_BR |
Subject | Circulating cathodic antigen (POC-CCA) | pt_BR |
Subject | Kato-Katz | pt_BR |
Subject | Helmintex | pt_BR |
Subject | Brazil | pt_BR |
Embargo date | 2030-12-31 | |