Please use this identifier to cite or link to this item:
https://www.arca.fiocruz.br/handle/icict/45743
Type
ArticleCopyright
Open access
Collections
- IOC - Artigos de Periódicos [12491]
Metadata
Show full item record
EXTERNAL FEMALE GENITALIA OF TRIATOMA JATAI, TRIATOMA COSTALIMAI AND TRIATOMA WILLIAMI (HEMIPTERA: REDUVIIDAE: TRIATOMINAE)
Author
Affilliation
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Vigilância Entomológica em Diptera e Hemiptera. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Vigilância Entomológica em Diptera e Hemiptera. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz Piauí. Teresina, PI, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Vigilância Entomológica em Diptera e Hemiptera. Rio de Janeiro, RJ, Brasil.
Centro Nacional de Diagnóstico e Investigación en Endemo‑Epidemias (CeNDIE), Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos Malbrán” (ANLIS). Ciudad Autónoma de Buenos Aires, Argentina / Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Ciudad Autónoma de Buenos Aires, Argentina.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Vigilância Entomológica em Diptera e Hemiptera. Rio de Janeiro, RJ, Brasil / Universidade Iguaçu. Departamento de Ciências Biológicas. Nova Iguaçu, RJ, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Vigilância Entomológica em Diptera e Hemiptera. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz Piauí. Teresina, PI, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Vigilância Entomológica em Diptera e Hemiptera. Rio de Janeiro, RJ, Brasil.
Centro Nacional de Diagnóstico e Investigación en Endemo‑Epidemias (CeNDIE), Administración Nacional de Laboratorios e Institutos de Salud “Dr. Carlos Malbrán” (ANLIS). Ciudad Autónoma de Buenos Aires, Argentina / Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Ciudad Autónoma de Buenos Aires, Argentina.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Vigilância Entomológica em Diptera e Hemiptera. Rio de Janeiro, RJ, Brasil / Universidade Iguaçu. Departamento de Ciências Biológicas. Nova Iguaçu, RJ, Brasil.
Abstract
Background: Taxonomic identification of triatomines is generally performed based on aspects of their external morphology.
However, the use of a multidisciplinary approach, considering morphological aspects of the external genitalia,
morphometry, genetics, and phylogeography has been suggested, especially for similar and/or cryptic species. The
rupestral species Triatoma jatai Gonçalves et al., 2013, Triatoma costalimai Verano & Galvão, 1959 and Triatoma williami
Galvão et al., 1965, which are morphologically similar, have been found naturally infected with Trypanosoma cruzi
(Chagas, 1909) in wild, peridomestic, and intradomestic environments, representing a risk of new outbreaks of Chagas
disease. This study presents morphological description complementation of these species, with an emphasis on the
structures of the female external genitalia, using scanning electron microscopy.
Methods: The females of T. jatai and T. costalimai (n = 10 of each) were captured in the Brazilian municipalities of
Paranã and Aurora do Tocantins and were identified with the use of a dichotomous key for the Matogrossensis subcomplex.
Females of T. williami (n = 5), were obtained from a laboratory colony. The females were cut transversely at
the sixth abdominal segment and examined under scanning electron microscopy (SEM) at the Oswaldo Cruz/Fiocruz
Institute Electronic Microscopy Platform.
Results: It was possible to differentiate the three species based on the characteristics of urotergites VII, VIII and IX and
urosternite VII, as well as the genital plaques, gonocoxites, and gonapophyses. To our knowledge, morphological differences
in the spines present on gonapophysis 8 in triatomines are described here for the first time.
Conclusions: The results show that external genitalia of females are useful structures to differentiate T. costalimai,
T. jatai and T. williami. SEM analysis contributes to and corroborates, together with other tools morphological and
molecular, the distinction of the three species.
Share